高效液相色谱法测定蜜枇杷叶苦杏仁苷含量  

Determination of Amygdalin in Honeyed Folium Eriobotryae by HPLC

作　　者：张瑾[1] 聂诗明[2] 

机构地区：[1]武汉大学中南医院药学部,湖北武汉430071 [2]湖北中医药大学药学院,湖北武汉430065

出　　处：《湖北中医药大学学报》2016年第2期32-34,共3页Journal of Hubei University of Chinese Medicine

基　　金：国家中医药管理局中医药行业专项(项目编号:201007012-2)

摘　　要：目的建立蜜枇杷叶中苦杏仁苷的高效液相色谱（HPLC）测定方法。方法 Welch Ultimate XB-C18（4.6mm×250mm,5μm）为固定相,甲醇-水（20∶80）为流动相,流速1.0 m L/min,检测波长210nm。结果苦杏仁苷在9.45~151.2μg/m L线性关系良好,r=0.9994。蜜枇杷叶中苦杏仁含量2.89%~3.11%。结论本方法分离度、线性关系、重现性、稳定性、回收率均符合含量分析要求,适合于蜜枇杷叶中苦杏仁苷的HPLC测定。Objective To establish a method for determing amygdalin in honeyed folium eriobotryae by HPLC. Methods Take Welch Ultimate XB- C18（ 4. 6 × 250 mm,5μm） as solid phrase,methanol- water（ 20∶ 80） as mobile phrase to detect the content at UV wavelength of210 nm. Results The liner range of amygdalin was 9. 45 ~ 151. 2μg / m L（ r = 0. 9994）,the average recovery of sample was 97. 06%. Conlusion This method is simple and its resolution,linear relationship reproducibility,stability and recovery rate all meet the requirements of content analysis,can be used as a determination method of amygdalin in honeyed Folium Eriobotryae.

关 键 词：蜜枇杷叶 苦杏仁苷 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]