红霉素高产菌株的推理选育  

Rational Selection of Erythromycin High- producing Strains

作　　者：万丹[1,2] 秦宝福[1] 刘迎宾[1,2] 张明明[1,2] 董维平[1,2] 李强[1,2] 

机构地区：[1]西北农林科技大学,陕西西安712100 [2]天方药业有限公司,河南驻马店463000

出　　处：《山东化工》2016年第8期65-67,共3页Shandong Chemical Industry

摘　　要：推理选育是一种应用广泛的高通量筛选技术,本文以红霉素生产菌红色链霉菌HA-13-36为出发菌株,经过氯化锂、紫外线、DES三重复合诱变后,定向筛选耐丙酸盐高产突变株Ⅰ(B-14-30),耐红霉素高产突变株Ⅱ(H-14-05),再利用原生质体融合技术将Ⅰ和Ⅱ融合后获得新的高产融合子R-15-26,该重组子摇瓶效价为6532u/m L,比原始菌株提高了36.2%,比诱变菌株提高23.9%,比高产突变菌株Ⅰ和Ⅱ分别提高了10.3%和16.0%,且经过多次传代表明具有较强的遗传稳定性,小试效价达到10000u/m L以上。Reasoning breeding is a kind of widely used high- throughput screening technology,based on the production of erythromycin bacterium Streptomyces red HA- 13- 36 for starting strain,after uv and lithium chloride and DES triple compound mutagenesis,directional filter resistance to sodium propionate high yield mutant strains Ⅰ（ B- 14- 30）,erythromycin resistance and high yield mutant strains Ⅱ（ H- 14- 05）,reuse of protoplast fusion technology of new production after merge theirⅠandⅡR- 15- 26,the reorganization of the son shake flask titer of 6532 u / m L,than the original strains increased by 36. 2%,23. 9% higher than that of mutation strains,than the high yield mutant strainⅠandⅡincreased by 10. 3% and 16. 0% respectively,and after many batches showed strong genetic stability,small titer reached more than 10000 u / m L.

关 键 词：红霉素 复合诱变 抗性筛选 原生质体融合 

分 类 号：Q93[生物学—微生物学]