猪流行性腹泻病毒的分离及其M基因的生物信息学分析  

Separation and Bioinformatics Analysis of PEDV M Gene

作　　者：代振江 梁海英[1] 曾智勇[1] 汤德元[1] 王伟丞 刘钊[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物疫病控制中心,贵州贵阳550025

出　　处：《贵州农业科学》2016年第4期28-30,共3页Guizhou Agricultural Sciences

基　　金：贵州省农业科技攻关项目"贵州省规模化猪场猪繁殖障碍性疫病防控体系的研究与示范"[黔科合NY字(2010)3042];贵州省动物疫病防控与兽医公共卫生保障科技创新人才团队[黔科合人才团队(2015)4016]

摘　　要：为了解贵州猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)的变异情况,对分离PEDV的M基因进行了克隆测序和生物信息学分析。结果表明:分离的PEDV株(用N-GZ表示)与贵州省动物疫病研究室曾报道的PEDV株(用GZ表示)位于同一进化分支,与疫苗株CV777处于不同进化分支。N-GZ株与GZ株M基因的核苷酸序列相似性为98.5%,表明其亲缘关系较近,与CV777疫苗株的亲缘关系稍远。多重比较发现,N-GZ株与CV777株M蛋白的抗原表位区存在3个氨基酸的差异,与GZ株相比存在2个氨基酸的突变。M基因是PEDV的保守基因,但N-GZ株M基因在抗原表位区存在一定程度突变。In order to know the mutation of Guizhou PEDV,the M gene was cloned,sequenced and analyzed by bioinformatics software in this study.Results：Phylogenetic tree showed that the current separated PEDV strain（N-GZ）and the previous separated PEDV（NG）were in the same branch,the NGZ and the vaccine strain CV777 were in the different branches.The similarity of the N-GZ and GZ was98.5%,it suggested that the two PEDV strains appeared in two different pig farms were the same PEDV strain.The result of sequence alignment showed that there were three amino acids to be different in the M protein epitope domain of N-GZ and CV777 and two amino acids to be different with GZ.The M gene is a conserved gene of PEDV,but a certain extent mutation appeared in epitope domain of M gene from N-GZ strain.

关 键 词：PEDV M蛋白 变异 生物信息学分析 

分 类 号：S828[农业科学—畜牧学]