生物降解菌MZS1对大芥菜及土壤中毒死蜱的降解效果  

Effect of Applying Bacillus subtilis MZS1 Strain on Degradation of Chlorpyrifos Residue in Brassica juncea and Soil

作　　者：黄春萍[1,2] 李江[1] 李艳琳[3] 伍君华[1] 王静[1] 李琪[1,2] 

机构地区：[1]四川师范大学生命科学学院,四川成都610068 [2]农田生态服务能力建设四川省高校工程中心,四川成都610068 [3]四川省农业科学院农产品加工研究所,四川成都610066

出　　处：《贵州农业科学》2016年第4期66-70,共5页Guizhou Agricultural Sciences

基　　金：四川省高等学校工程中心开放课题基金项目"农田生态服务能力建设"(2014-GCZH-03);四川省教育厅课题"高山森林溪流中的微生物群落变化"(15ZB0031);四川师范大学开放项目"毒死蜱降解微生物的筛选及应用"(KF2015017);四川师范大学大精开放基金项目(DJ2015-1;DJ2015-14)

摘　　要：为探明生物降解菌对有机磷类农药残留的降解效果,丰富毒死蜱残留的生物降解菌菌种资源,采用传统方法和分子鉴定方法对实验室分离获得的毒死蜱降解菌株进行鉴定,并采用室内模拟与田间试验相结合的方法研究该菌株对大芥菜及土壤中毒死蜱残留的生物修复作用。结果表明:该菌株为枯草芽孢杆菌MZS1(Bacillus subtilis MZS1)。室内,施用菌株MZS1处理15d后其对土壤中浓度为5 mg/kg和10mg/kg毒死蜱的降解率分别为49.64%和53.02%;田间,随含菌量的增加,菌株MZS1对大芥菜和土壤中毒死蜱的降解率增加,其中,施用量为5L/hm2时,15d后其对大芥菜和土壤中毒死蜱的降解率分别为33.27%和42.93%。菌株MZS1对大芥菜的生长具有一定的促进作用。The degradation strain against Chlorpyrifos was isolated and identified by the traditional and molecular identification methods and the bioremediation effect of the strain on chlorpyrifos residue in B.junceaand soil was studied by indoor simulation and field tests to explore the degradation efficiency of biodegradable bacteria against the residue of organophosphorus pesticides and enrich biodegradable bacteria resources against Chlorpyrifos residue.Results：The strain is B.subtilis MZS1.The degradation rate of chlorpyrifos in soils applying with 5mg/kg and 10mg/kg MZS1 is 49.64% and 53.02% after 15 din the indoor simulation test respectively.The degradation rate of chlorpyrifos in B.juncea and soil increases with increase of MZS1 application amount in the field test and the degradation rate of chlorpyrifos in B.junceaand soil is 33.27% and 42.93% after 15 dseparately when MZS1 application amount is 5L/hm2.The MZS1 strain has a certain promotion effect on growth of B.juncea.

关 键 词：芽孢杆菌 毒死蜱 降解 生物修复 大芥菜 土壤 

分 类 号：S182[农业科学—农业基础科学]