艾纳香油对小鼠耳肿胀的抗炎效果  

Anti-inflammatory Effect of Blumea balsamifera Oil Against Mouse Ear Edema

作　　者：马青松[1,2,3] 王丹[2,3] 庞玉新 杨全[1] 李小婷[1,2,3] 许罗凤[1,2,3] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点开放实验室,海南儋州571737 [3]海南省艾纳香工程研究中心,海南儋州571737

出　　处：《贵州农业科学》2016年第4期100-102,共3页Guizhou Agricultural Sciences

基　　金：贵州省重大科技专项计划项目[黔科合重大专项字(2013)6011];海南省科技合作专项(6011;2013);海南省科技合作专项"热带药用植物种质资源评价;保护和开发利用"(KJHZ2015-15)

摘　　要：为了解艾纳香油的抗炎作用机理,为其开发应用提供参考,将60只昆明种小鼠(SPF级)随机分为艾纳香油高(40%)、中(20%)、低(10%)剂量组,氢化可的松组,模型对照组和溶剂对照组,用二甲苯致炎,采用紫外可见分光光度法测定致炎耳组织中前列腺素E2(PGE2)和血清中丙二醛(MDA)的含量,并采用超氧化物歧化酶(SOD)试剂盒测定血清SOD活性。结果表明:与模型对照组相比,艾纳香油高、中、低剂量组对二甲苯所致的小鼠耳肿胀均有显著抑制作用(P<0.05),降低PGE2含量,其中低剂量组可极显著降低致炎耳组织中PGE2含量(P<0.01);艾纳香油高、低剂量组均显著提高血清SOD的活性(P<0.05),艾纳香油可降低血清MDA含量(P>0.05)。艾纳香油具有一定的抗炎作用,可能与降低炎症区域PGE2含量和提高机体抗氧化损伤有关。60SPF Kunming mice were randomly divided into 40% BBO group,20% BBO group,10%BBO group,hydrocortisone group,mode control group and solvent control group.PGE2 and serum MDA content of the inflamed ear tissues was determined by UV spectrophotometry and the serum SOD activity was detected by SOD activity assay kit to discuss the anti-inflammatory mechanism of B.balsamifera oil（BBO）and provide a reference for development and application of B.balsamifera oil（BBO）.Results：B.balsamifera oil（BBO）has the significant inhibition effect on mouse ear edema treated with xylene compared with the mode control group（P〈0.05）and reduces its PGE2 content.BBO in 10% BBO group reduces PGE2 content in the inflamed ear tissues very significantly.BBO in 40% BBO group and 10% BBO group increases serum SOD activity（P〈0.05）and reduces serum MDA content（P〈0.05）.BBO is of a certain anti-inflammatory action,which may be related to reducing PGE2 content in inflammation area and improving the body＇s anti-oxidative damage.

关 键 词：艾纳香油 耳肿胀 抗炎 前列腺素E2 氧自由基 

分 类 号：S853.7[农业科学—临床兽医学]