莪术醇对结直肠癌细胞裸鼠移植瘤生长及其VEGF和COX-2表达的影响  

Effects of Curcumol on Growth of Xenograft Tumors and Expression of VEGF and COX-2 in Nude Mice with Colorectal Cancer

作　　者：池碧霞 王娟[1] 白准 胡雷蕾 严威[1] 李旭梅[1] 武家才[1] 陈旭[1] 

机构地区：[1]桂林医学院,广西桂林541004 [2]株洲市中心医院,湖南株洲412007

出　　处：《中国实验方剂学杂志》2016年第8期121-125,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科学研究与技术开发项目(1355001-5-7);桂林市科学研究与技术开发项目(20140105-8);广西医药产业人才小高地项目(1415)

摘　　要：目的:观察莪术醇对人结直肠癌Lo Vo细胞裸鼠移植瘤的抑制作用,探讨其可能的机制。方法:建立人结直肠癌Lo Vo细胞裸鼠移植瘤模型,成瘤后将其随机分为莪术醇高、中、低剂量(80,40,20 mg·kg-1)组,模型组,空白组,每天ig给药1次,给药21 d。用逆转录聚合酶链反应(RT-PCR)方法和免疫组化方法(IHC)检测瘤组织中血管生成因子(VEGF)、环氧合酶-2(COX-2)的表达。结果:与模型组比较,莪术醇高、中、低剂量组能明显降低肿瘤的瘤体积、瘤重及VEGF,COX-2的表达,均呈剂量依赖性。莪术醇高、中、低剂量组抑瘤率分别为66.88%,42.08%,25.73%;模型组VEGF表达量与莪术醇各组比较,差异有统计学意义(P<0.05);模型组COX-2表达量与莪术醇中、高剂量组比较,差异具有统计学意义(P<0.05),VEGF和COX-2表达具有明显相关性(r=0.874,P<0.01)。结论:莪术醇抑制结直肠癌Lo Vo细胞裸鼠移植瘤生长的机制可能与下调VEGF和COX-2表达有关,并且可能通过COX-2/前列腺素E2(PGE2)/VEGF信号通路发挥抑制结直肠癌生长的作用。Objective： To evaluate the effects of curcumol on Lo Vo cells-planted xenogaft tumors in nude mice with colorectal cancer（ CRC） and to investigate the possible mechanism. Method： Human CRC Lo Vo cells were implanted in BALB / c nude mice,then,tumor-bearing mice were randomly divided into five groups： blank group,model group,curcumol low dose group,middle dose group and high dose group（ 20,40,80 mg·kg- 1,respectively）. The drugs were administered by intragastric injection（ 21 days）,then the expressions of vascular endothelial growth factors（ VEGF） and cyclooxygenase-2（ COX-2） in these tumor tissues were determined by reverse transcription-PCR（ RT-PCR） and immunohistochemistry. Result： Compared with the model group,curcumol high dose group,middle dose group and low dose group significantly inhibited the tumor volume,tumor weight,and expressions of VEGF and COX-2 in a dose-dependent manner. The anti-tumor rate was 25. 73%,42. 08%,66. 88% respectively in curcumol low dose group,middle dose group and high dose group. There was statistical difference in expression of VEGF between model group and all curcumol groups（ P 〈 0. 05）. There was statistically significant difference in COX-2 expression between model group and curcumol middle and high dose groups（ P 〈 0. 05）. Moreover,VEGF and COX-2 expressions were positively correlated with each other（ r =0. 874,P 〈 0. 01）. Conclusion： Curcumol significantly inhibited tumor growth in CRC implanted mice,probably associated with down-regulating expressions of VEGF and COX-2,and may have a role in treating human colorectal cancer by the signal pathways of COX-2 / PGE2/ VEGF.

关 键 词：莪术醇 结直肠癌LoVo细胞 裸鼠移植瘤 血管生成因子 环氧合酶-2 

分 类 号：R285.5[医药卫生—中药学]