三七总黄酮对高血脂大鼠血脂的影响  

Effect of Total Flavonoids from Notoginseng Radix et Rhizoma in Reducing Blood Lipid

作　　者：赵文萃[1] 张宁[1] 周慧琴[1] 刘春梅[1] 张琦[1] 

机构地区：[1]中国人民解放军第二0八医院,长春130000

出　　处：《中国实验方剂学杂志》2016年第8期143-147,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：“十一五”国家科技支撑计划重点项目(2006BAI06A05);吉林省科技厅项目(20130302026YY)

摘　　要：目的:研究三七总黄酮的降血脂作用及机制。方法:Wistar大鼠建立高血脂动物模型,随机分为正常组,高血脂模型组,三七总黄酮高、中、低剂量组(200,100,50 mg·kg-1),辛伐他丁组(8 mg·kg-1),连续ig给药6周,生化法检测大鼠血液和肝脏组织中总胆固醇(TC),甘油三酯(TG),高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL-C)的含量。采用脂肪乳诱导肝L-02细胞脂肪变性,分为正常组、模型组、三七总黄酮低、中、高剂量组(100,300,500μmol·L-1)和辛伐他汀(100μmol·L-1)组,共培养24 h,生化法测定TG和TC含量,酶联免疫吸附测定(ELISA)法检测二脂酰甘油酰基转移酶(DGAT),羟甲基戊二酸单酰辅酶(HMG-Co A),胆固醇7α-羟化酶(CYP7A),肝脏甘油三脂脂肪酶(HTGL)含量。结果:大鼠实验中,与正常组比较,模型组大鼠的血脂升高(P<0.01);与高血脂模型组比较,三七总黄酮高剂量能抑制大鼠肝脏指数(P<0.05),高、中剂量能明显降低血清TC,TG,LDL-C含量(P<0.05,P<0.01),提高HDL-C含量(P<0.05,P<0.01),并且能够抑制大鼠肝组织TC,TG的含量(P<0.05)。细胞实验中,三七总黄酮中、高剂量可明显降低脂肪化细胞TC,TG含量(P<0.05,P<0.01);降低DGAT,HMG-Co A活性(P<0.01);明显升高CYP7A,HTGL活性(P<0.05,P<0.01)。结论:三七总黄酮具有较好的降血脂作用,可能与其降低DGAT,HMG-Co A活性,升高CYP7A,HTGL活性有关。Objective： To study total flavonoids from Notoginseng Radix et Rhizoma（ FN） in reducing blood lipid and its mechanism. Method： Hyperlipidemia animal model was set up in Wistar rats,and randomly divided into normal control group,hyperlipidemia model group,FN high,medium and low dose groups（ 200,100,50 mg·g- 1）,and Simvastatin butyl group（ 8 mg·kg- 1）. They were orally given drugs for six weeks.Biochemical method was used to detect totalcholesterol（ TC）, triglyceride（ TG）, low-density lipoprotein cholesterol（ LDL-C） and high-densty lipoprotein cholesterol（ HDL-C） content in rat blood and liver tissues. The steatosis model was established by exposing L-02 hepatocytes in fat emulsion,randomly divided into normal control group,model group,FN low,medium and high dose groups（ 100,300,500 μmol·L- 1）,and Simvastatin butyl group（ 100 μmol·L- 1）,and cultured for 24 h. The effects of FN on the contents and activities of key enzymes and proteins in the synthesis and decomposition pathways of TG and TC were investigated by biochemical method;ELISA was used to detect diacylgcerol acyltransferase（ DGAT）,3-hydroxy-3-methylglutaryl-coenzyme（ HMGCo A）,cholesterol 7a-hydroxylase（ CYP7A） and lipase liver triglycerides（ HTGL） contents. Result： In the rat experiment,compared with the normal group,the model group showed a higher blood lipid（ P 〈 0. 01）; compared with the hyperlipidemia model group,FN middle dose and high dose（ 300,500 μmol·L- 1） groups inhibited rat liver weight（ P 〈 0. 05）,reduced TC,TG,LDL-C content in serum（ P 〈 0. 05,P 〈 0. 01）,increased HDL-C content（ P 〈 0. 05,P 〈 0. 01）,and inhibited concentrations of TC and TG in liver（ P 〈 0. 05）. In the cell experiment,FN middle dose and high dose groups can significantly reduced the levels of TC,TG（ P 〈 0. 05,P 〈0. 01） and DGAT,HMG-Co A activity（ P 〈 0. 01）,and increased CYP7 A,HTGL activity（ P 〈 0. 05, P 〈0. 01）. Conclusion： FN can reduce blood lipi

关 键 词：三七总黄酮 高血脂大鼠 人肝L-02细胞 血脂 

分 类 号：R285.5[医药卫生—中药学]