夏枯草提取物对AHF模型大鼠心肌保护作用及SDF-1表达水平的影响  

Protective Effects of Prunellae Spica Extract on Myocardium and Its Effect on Expression of SDF-1 in AHF Rat Model

作　　者：刘宏扬 张睿 任公平[2] 

机构地区：[1]齐齐哈尔市中医医院,黑龙江齐齐哈尔161000 [2]牡丹江医学院红旗医院,黑龙江牡丹江157011

出　　处：《中国实验方剂学杂志》2016年第8期171-176,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黑龙江省中医药管理局项目(ZHY14-W76)

摘　　要：目的:探讨夏枯草提取物对急性心力衰竭(AHF)模型大鼠心肌保护作用及基质细胞衍生因子-1(SDF-1)表达的影响,探讨其治疗AHF的可能作用机制。方法:采用Wistar大鼠建立AHF模型,随机分为4组,AHF模型组(AHF组),给予等体积生理盐水ig;夏枯草低剂量组,按照夏枯草提取物0.5 g·kg-1剂量ig;夏枯草中剂量组,按照夏枯草提取物1.0 g·kg-1剂量ig;夏枯草低剂量组,按照夏枯草提取物2.0 g·kg-1剂量ig;另设假手术组(Sham组),给予等体积生理盐水ig。6周后进行血流动力学、超声心动图测量,并分别采用流式细胞术,苏木素-伊红(HE)染色测量CD34+细胞阳性率,微血管密度(MVD)。进一步采用蛋白质免疫印迹(Western blot)和实时荧光定量-聚合酶链式反应(q PCR)分析检测SDF-1蛋白和SDF-1mRNA表达的变化。结果:夏枯草提取物可有效改善AHF大鼠左心室舒张功能,减轻心室壁厚度,促进CD34+细胞的动员和新生血管的形成,与AHF组比较,差异有统计学意义(P<0.05,P<0.01)。夏枯草提取物可使SDF-1蛋白和SDF-1 mRNA的表达显著增加,与AHF组比较,差异有统计学意义(P<0.05,P<0.01)。结论:夏枯草提取物对衰竭心脏有明显的保护作用,其机制可能与促进新生血管的形成,升高SDF-1表达水平和动员CD34+细胞释放至外周血有关。Objective： To investigate the protective effect of Prunellae Spica extract on the myocardium and its effect on the expression of stromal cell derived factor-1（ SDF-1） in acute heart failure（ AHF） rat models and discuss its possible mechanism. Method： Wistar rats were used to establish AHF models and randomly divided into four groups, such as AHF model group（ AHF group） with equal volume of normal saline by intragastric administration; Prunellae Spica low-dose group,0. 5 g·kg- 1Prunellae Spica extract by intragastric administration; Prunellae Spica middle-dose group, 1. 0 g ·kg- 1Prunellae Spica extract by intragastric administration; and Prunellae Spica high-dose group, 2. 0 g ·kg- 1Prunellae Spica extract by intragastric administration. Another sham operation group（ Sham group） was set to receive equal volume of normal saline by intragastric administration. After 6 weeks,hemorheology examination and ultrasonic cardiogram were taken. The positive rate of CD34＋cells and microvessel density（ MVD） were measured by flow cytometry and HE staining respectively. Western blot and q PCR were further used to detect the protein and mRNA expressions of SDF-1.Result： Prunellae Spica extract can effectively improve left ventricular diastolic function,reduce ventricular wall thickness and promote formation of CD34＋cell mobilization and angiogenesis in AHF rat models,with statistically significant difference as compared with AHF group（ P 〈 0. 05, P 〈 0. 01）. Prunellae Spica extract can significantly increase the expressions of SDF-1 protein and SDF-1mRNA,with statistically significant difference as compared with AHF group（ P 〈 0. 05,P 〈 0. 01）. Conclusion： Prunellae Spica extract has obvious protective effect on heart failure,and the mechanism may be related to promoting angiogenesis,increasing SDF-1 expression levels and mobilizing the release of CD34＋cells to peripheral blood.

关 键 词：夏枯草提取物 急性心力衰竭 基质细胞衍生因子-1 

分 类 号：R285.5[医药卫生—中药学]