广东省猪流行性腹泻病毒S1基因的克隆与序列分析  

Cloning and Sequence analysis of S1 genes of porcine epidemic diarrhea virus in Guangdong Province

作　　者：罗六龙 黄冬妮[1,2] 黄良宗[2] 马春全[2] 梁梓森[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]佛山科学技术学院,广东佛山528231

出　　处：《中国兽医杂志》2016年第3期19-21,共3页Chinese Journal of Veterinary Medicine

基　　金：广东省现代生猪产业体系项目;广东高校畜禽疾病诊断防控技术开发中心(GCZX-B1102);广东省省级科技计划项目(2012B020202002)

摘　　要：为研究广东省猪流行性腹泻病毒S1基因的变异情况,于2012-2013年间从广东省不同地区猪场采集56份腹泻病仔猪样本。通过PCR方法扩增到12个样本的PEDV的S1基因序列,并进行序列比对及遗传分析。结果 12个样本PEDV的S1基因序列之间的核苷酸同源性为91.1%-99.6%,氨基酸的同源性为88.5%-99.1%。12个样本PEDV的S1基因序列与国内参考毒株核苷酸的同源性为91.0%-99.9%,氨基酸的同源性为88.5%-99.7%。与国外参考毒株核苷酸的同源性为89.0%-99.7%,氨基酸的同源性为86.5%-99.4%。与经典毒株CV777的核苷酸同源性为91.0%-100.0%,氨基酸的同源性为88.3%-99.9%。其中,仅GD-HY的S1基因与CV777的同源性达100.0%,其他同源性较低。表明近年广东省的PEDV流行毒株以变异株为主,与疫苗株（CV777）的亲缘关系较远。To investigate the S1 genetic variation of porcine epidemic diarrhea virus in Guangdong Province,56 piglet diar-rhea samples were collected from swine farms in different areas.S1 gene sequences of 12 PEDV strains were amplified by PCR andcompared with different strains to analyze the genetic characteristics and evolution regularity. Result showed that the 12 S1 genesshared 91.1%-99.6% nucleotide homology and 88.5%-99.1% amino acid homology.The 12 S1 genes shared 91.0%-99.9% nu-cleotide homologyand 88.5% -99.1% amino acid homology with Chinese reference strains. The 12 S1 genes shared 89.0% -99.7% nucleotide homology and 86.5% -99.4% amino acid homology with foreign reference strains. The 12 S1 genes shared91.0%-100.0% nucleotide homology and 88.3%-99.9% amino acid homology with classic strain CV777. Only GD-HY shared100.0% homology with classic strain CV777,while the rest of the 12 S1 genes shared lower homology with CV777.The results impli-cate that variations of Guangdong PEDV strains have occurred in recent years,and they are distant with CV777.

关 键 词：猪流行性腹泻病毒 S1基因 序列分析 

分 类 号：S852.651[农业科学—基础兽医学]