牛口蹄疫O型合成肽VP1结构蛋白ELISA抗体检测方法的建立  

Development of an ELISA based on synthetic peptide for detection of cattle foot-and-mouth disease virus type O VP1 antibody

作　　者：张蕾 肖进[1,2] 董春娜 巴利民 栗利芳 宋芳 王楠 周强 齐鹏 汪明[1,2] 

机构地区：[1]中牧实业股份有限公司农业部兽用生物制品与化药重点实验室北京市兽用多肽疫苗设计与制备工程技术中心,北京丰台100070 [2]中国农业大学动物医学院,北京海淀100193

出　　处：《中国兽医杂志》2016年第3期22-24,共3页Chinese Journal of Veterinary Medicine

摘　　要：根据牛口蹄疫O型3个拓扑型VP1序列设计3条合成肽,以此为包被抗原建立牛口蹄疫O型合成肽VP1结构蛋白ELISA抗体检测方法,并对该方法敏感性、特异性和重复性进行验证。结果显示,该方法敏感性为96.7%,特异性为99.1%,批内和批间变异系数分别为2.1%-9.8%和3.5%-10.7%。与2种商品化试剂盒比较,符合率分别为93.5%和85.9%。结果表明,该方法敏感性好、特异性强、稳定性高,可用于牛口蹄疫O型抗体水平检测。Three synthetic peptides of FMDV VP1 were used to detect antibody of FMDV type O.The ELISA system was optimized and the sensitivity,specificity and repeatability of this method were tested.The results showed that the sensitivity and specificity were 96.0% and 99.1%,and variations of intra-and inter-assay repeatability were 2.1%-9.8% and 3.5%-10.7%.Comparing this method with 2 test kits showed a coincidence rate of 93.5% and 85.7%.The results show that this method has high sensitivity and specificity,and is stable,which can be used to monitor VP1 antibody.

关 键 词：牛 口蹄疫 合成肽 ELISA 

分 类 号：S858.23[农业科学—临床兽医学]