鸡大肠杆菌宽谱噬菌体的分离及裂解性研究  

The Study on Isolation and Lysis of Wide Spectum Phage of Chicken Escherichia coli

作　　者：张晓冬[1] 李冰[1] 汲晨 卢赫[1] 冯方周 

机构地区：[1]辽宁医学院畜牧兽医学院,辽宁锦州121001

出　　处：《中国兽医杂志》2016年第3期38-40,共3页Chinese Journal of Veterinary Medicine

基　　金：2014年国家级大学生创新创业训练项目(201401-160024);辽宁省科技厅计划项目(2013301003)

摘　　要：为了解鸡宽谱大肠杆菌噬菌体的裂解特性,本试验以30株大肠杆菌为宿主菌,从8份粪便中成功分离出5个噬菌体,并从中筛选出1个强裂解性宽谱大肠杆菌噬菌体,命名为Z-SN5,宽噬率为70%,热稳定性相对良好,60℃作用1 h,尚有活性;耐碱性强于耐酸性,在pH值=7时活性最高,经PCR鉴定后,成功扩增出目的基因片段。本试验为今后研究噬菌体治疗鸡大肠杆菌病奠定基础。In order to learn about the cleavage characters of the broad spectrum colibacteriophage of chickens,we chose 30 strains of E.coli as the host bacteria and successfully extract five strains of bacteriophages from the 8 groups of faeces in this experiment. Then,a strong broad spectrum colibacteriophage was selected from bacteriophages and named Z-SN5. Its broad phagocytic rate was 70%,and the thermostability was quite well.It was still active after reaction at 60 ℃ for 1 h.Its alkali resistance was better than its acid resistance,and the activity was highest when the PH was 7. After Identification with PCR many target gene segmentscould be amplified.This study lays a foundation for studying how to cure colon bacillus diseases by bacteriophage in future.

关 键 词：鸡 大肠杆菌 宽谱噬菌体 裂解性 

分 类 号：S858.31[农业科学—临床兽医学]