花生^(60)Co-γ辐射诱变和突变体库的构建  

^(60)Co-γ Radiation and Construction of Mutant Bank of Arachis hypogeae L.

作　　者：彭振英[1] 王兴军[1] 田海莹 郑玲[1] 单雷[1] 范仲学[1] 边斐[1] 郭峰[1] 王莹莹[1] 万书波[1] 

机构地区：[1]山东省农业科学院生物技术研究中心/山东省作物遗传改良与生态生理重点实验室,山东济南250100

出　　处：《核农学报》2016年第3期422-429,共8页Journal of Nuclear Agricultural Sciences

基　　金：山东省自然科学基金(ZR2013CM036);山东省农业良种工程项目

摘　　要：为了获得花生新种质,对花生干种子进行60Co-γ辐射诱变处理并构建花生突变体库,获得了一些有价值的突变体材料,如高油突变体、高蛋白突变体、大种子和小种子突变体、大叶和小叶突变体、卷叶突变体等。通过分析这些突变体的不同性状发现,辐射处理对株高、叶片形态、含油量、蛋白含量、单株结荚数、单粒重、油亚比、出仁率等性状的影响极为显著。对7个性状(单株果仁重、蛋白含量、亚油酸含量、油酸含量、含油量、单粒重和单株荚果重)进行相关性分析,结果表明,油酸含量与亚油酸含量为高度线性相关;含油量与蛋白含量、亚油酸含量、油酸含量均为显著性相关;而含油量与单株荚果产量呈低度线性相关。这些突变体的获得为今后进行花生遗传育种以及机理研究提供了可靠的原材料和中间材料,也为花生功能基因组学研究提供了丰富的材料。In order to obtain new peanut materials,a peanut mutant bank was conducted using^60-radiation on peanut dry seed. A lot of valuable mutants,such as high-oil content,high-protein content,large-seed,small-seed,large-leaf,small-leaf,and curly-leaf mutants were obtained. The M2 and M3generation were analyzed and It＇s found that^60- γradiation greatly affected the height,leaves,oil-content,protein-content,podding number,single grain weight,O / L ratio and kernel rate. The correlation analysis on seven traits（ seeds weight,protein content,linoleic acid content,oleic acid content,oil content,single seed weight and pod weight）,showed that there existed high-correlations between linoleic acid content and oleic acid content,oil content and linoleic acid content,oleic acid content.,whereas oil content and protein content showed high negative correlation. Oil content and pod weight showed low correlation. These mutants are excellent materials for peanut genetic breeding and mechanism study,also for peanut functional genomics researches.

关 键 词：花生 突变体 辐射诱变 相关性分析 

分 类 号：S565.2[农业科学—作物学]