基于转录组学测序的长白落叶松材性表达基因  

Gene Expression of Wood Properties Based on Transcriptome of Larix olgensis

作　　者：赵佳丽[1] 张磊[1] 张素芳[1] 王艳红[1] 张含国[1] 

机构地区：[1]林木遗传育种国家重点实验室(东北林业大学),哈尔滨150040

出　　处：《东北林业大学学报》2016年第4期8-12,共5页Journal of Northeast Forestry University

基　　金：国家高技术研究发展计划(863计划)(2013AA102704)

摘　　要：基于边合成边测序(Sequencing By Synthesis,SBS)技术,使用Illumina Hi Seq2500高通量测序平台对长白落叶松c DNA文库进行测序。共获得非重复序列基因58 683条,总长度为55 283 938 bp。将得到的转录本使用BLAST软件将非重复序列基因序列与NR、Swiss-Prot、GO、COG、KEGG数据库比对,通过选择BLAST参数Evalue不大于10-5,最终获得29 350个有注释信息的基因序列,其中19 292个转录本注释GO编号,有9 112个转录本在COG数据库中被注释并分为25个功能分类,同时注释到120条KEGG代谢途径。在得到注释的转录本中搜索到木质素相关基因共68条,其中与木质素合成相关基因29条,与木质素分解代谢相关基因27条;找到了147条纤维素相关基因,其中与纤维素合成相关基因103条,与纤维素分解代谢相关基因44条。Based on sequencing-by-synthesis（ Sequencing By Synthesis,SBS） technology,we used Illumina HiS eq2500 highthroughput sequencing to perform the cDNA library Larix olgensis for sequencing platform. We acquired 58 683 Unigenes,and the total length was 55 283 938 bp. We used BLAST software to compare Unigenes sequence of transcript with NR,Swiss-Prot,GO,COG,KEGG database.The parameter of E-value was not more than 10-5with 29 350 annotated Unigenes.Among them,19 292 annotations was from GO,9 112 from COG,and these Unigenes was divided into 25 functional categories,120 from KEGG. There were 68 Unigenes connected with lignin biosynthesis in the annotated transcripts. The Unigenes related to lignin biosynthesis was 29,and the Unigenes related to lignin catabolism was 27. We found 147 Unigenes related to cellulose. The Unigenes related to cellulose biosynthesis was 103,and the Unigenes related to cellulose catabolism was 44.

关 键 词：长白落叶松 转录组 高通量测序 材性基因 

分 类 号：S791.225[农业科学—林木遗传育种]