山西西葫芦花叶病病原鉴定与部分序列分析  

Identification and partial sequence analysis of the pathogen causing Cucurbita pepo L.mosaic disease in Shanxi province

作　　者：王德富[1] 时晓丽[1] 寇丽莎 庞小静[1] 刘勇 牛颜冰[1] 

机构地区：[1]山西农业大学生命科学学院,山西太谷030801 [2]湖南省植物保护研究所,湖南长沙410125

出　　处：《山西农业大学学报（自然科学版）》2016年第5期310-314,363,共6页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：公益性行业(农业)科研专项(No.201303028);山西农业大学科技创新基金(20142-10);山西农业大学引进人才科研启动基金(2014YJ05)

摘　　要：利用双链RNA（double-stranded RNA,dsRNA）技术和非序列依赖PCR扩增（sequence-independent amplification,SIA）方法对感病西葫芦进行了分子鉴定。序列测定及分析发现,具有明显花叶和斑驳症状的西葫芦受到黄瓜花叶病毒（Cucumber mosaic virus,CMV）的侵染。为进一步明确黄瓜花叶病毒山西西葫芦分离物（CMV-XHL）的分类地位,克隆了CMV-XHL RNA3的外壳蛋白和移动蛋白基因全序列。通过序列比对分析,发现CMV-XHL CP核苷酸序列和氨基酸序列与CMV亚组ⅠB中CMV烟草分离物（CMV-SG）的相似性最高,分别为98.6%和99.5%;MP核苷酸序列和氨基酸序列与CMV亚组Ⅰ分离物的相似性最高,为93.2%~94.6%。MP和CP氨基酸序列系统进化分析表明,CMV-XHL与中国大多数CMV分离物聚为一类,属于CMV亚组Ⅰ中的成员。研究结果对西葫芦病毒病的防治提供一定的理论依据。To identify the viruses that induced Cucurbita pepo L.mosaic disease,dsRNA technology and sequence-independent amplification（SIA）were used.Results of SIA and sequencing showed that Cucurbita pepo L.was infected by Cucumber mosaic virus（CMV）.To further characterize the Shanxi isolate of Cucumber mosaic virus（CMV-XHL）,the coat protein gene（CP）and movement protein gene（MP）coded by CMV-XHL RNA3 were cloned and sequenced.Sequence alignments showed that CMV-XHL has the highest identities of 98.6%in nucleotide level and 99.5%in deduced amino acid level with the strain SG which belong to CMV subgroupⅠB and 93.2%~94.6%in CMV SubgroupⅠ.Phylogenetic analysis of CP and MP suggested that CMV-XHL belongs to CMV subgroupⅠ.The results of this study may serve as a theoretical basis to prevent and control Cucumber mosaic virus.

关 键 词：西葫芦 黄瓜花叶病毒 病原鉴定 序列分析 

分 类 号：S432.41[农业科学—植物病理学]