Ⅰ型钠氢交换蛋白调节细胞内酸碱平衡对胃癌细胞迁移、侵袭的影响  

Effect of NHE1-regulated intracellular pH on invasion and migration of gastric cancer cells

作　　者：谢睿[1] 王海波[1] 牟海军[1] 徐靖宇[1] 

机构地区：[1]遵义医学院附属医院消化内科,贵州遵义563099

出　　处：《中国现代医学杂志》2016年第6期6-10,共5页China Journal of Modern Medicine

基　　金：贵州省卫生计生委科学技术基金项目(No:gzwjkj2014-2-165)

摘　　要：目的观察I型钠氢交换蛋白(NHE1)通过调控胃癌细胞SGC-7901内酸碱值(p H)对其迁移、侵袭的影响。方法采用共聚焦显微镜观察比较用特异性阻断剂5-(N-乙基-N-异丙基)阿米洛利(EIPA)阻断胃癌细胞SGC-7901的NHE1功能前后胞内p H值的变化,划痕实验观察细胞迁移能力的改变,transwell侵袭实验观察细胞侵袭能力的改变。结果共聚焦实验发现对照组细胞内p Hi为(7.2±0.12),EIPA阻断剂组(5μm)为(7.01±0.23),EIPA阻断剂组细胞内的p H值明显降低(P<0.05);进一步划痕和侵袭实验证明加入不同浓度的EIPA(5、10和20μmol)后均能有效地抑制胃癌细胞SGC-7901的迁移和侵袭能力(P<0.05,P<0.01),并且抑制的程度与EIPA的浓度呈剂量依赖性。结论阻断NHE1可以降低胃癌细胞p H值,进而抑制胃癌细胞的迁移和侵袭能力。objective To observe the effect of Na＋/H＋exchanger 1（NHE1）-regulated intracellular p H on the invasion and migration of gastric cancer cell line SGC-7901. Methods The gastric cancer cell line SGC-7901 was treated with the NHE1 specific inhibitor 5-（N-ethyl-N-isopropyl） Amiloride（EIPA）, the intracellular p H of SGC-7901 was measured using confocal fluorescence microscope. The migration of SGC-7901 cells was examined by scratch test. The invasion of SGC-7901 cells was examined by transwell chamber invasive test. Results The intracellular p H in the control group was（7.2 ± 0.12）, it significantly reduced to（7.01 ± 0.23） in the 5-μmol EIPA group. EIPA of different concentrations（5 μmol, 10 μmol and 20 μmol） could inhibit the invasion and migration of SGC-7901 cells in a dose-dependent manner. Conclusions NHE1 plays an important role in the regulation of intracellular p H. Blockage of NHE1 could restrain the invasion and migration of gastric cancer cells.

关 键 词：NHE1 胃癌 细胞迁移 p H 细胞侵袭 

分 类 号：R975.6[医药卫生—药品]