药对研究——三七、白及药对配伍前后对三七皂苷成分含量的影响  

Research on Chinese medicine pairs effects of Panax notoginseng and Bletilla striata before and after compatibility on contents of notoginsenosides

作　　者：刘文君[1] 程宁波[1] 孟兆青[1,2] 费洪强[1] 孙林[1] 黄文哲[1] 王振中[1] 王跃生[1,3] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中国药科大学,江苏南京210009 [3]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2016年第5期887-890,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2013ZX09402203)

摘　　要：该文以三七、白及药对为研究对象,采用HPLC测定三七单煎液及不同配伍比例(1∶0.5,1∶1,1∶2)三七与白及合煎液中具有脂肪酶抑制作用的活性成分——(20S)-人参皂苷Rg3和人参皂苷Rh4的含量,并探讨两药配伍后对三七皂苷成分含量的影响。色谱方法为Lichrospher C18(4.6 mm×250 mm,5μm)色谱柱;流动相为乙腈-水梯度洗脱;检测波长为203 nm;柱温为30℃;流速为1 m L·min-1。结果发现,与三七单煎液相比,三七、白及合煎液中人参皂苷Rg3、人参皂苷Rh4的含量均呈上升趋势,且上升幅度与配伍白及量呈正比;合煎液中人参皂苷Rb1、人参皂苷Rg1、三七皂苷R1的色谱峰均较三七单煎液的明显降低。研究结果表明三七与白及配伍后,合煎液中(20S)-人参皂苷Rg3、人参皂苷Rh4的含量较单煎液显著增加,推测白及中的有关成分可促进其它人参皂苷进行转化,该结果为拓展制备(20S)-人参皂Rg3、人参皂苷Rh4的方法提供参考。To discuss the synergistic mechanism of compatible use of two medicinal herbs,Panax notoginseng and Bletilla striata,an HPLC was established to determine two ginseng saponins（ 20S）-ginseng saponin Rg3 and ginseng saponin Rh4 contained in single decoction of Panax notoginseng as well as in compound decoction of Panax notoginseng and Bletillastriata in different compatibility ratio（ 1∶ 0. 5,1∶ 1,1∶ 2）,followed by analyzing the impact of amount of notoginsenosides after compatibility. As a result,compared with the single decoction of Panax notoginseng,the contents of ginseng saponin Rg3 and ginseng saponin Rh4 in the compound decoction of Panax notoginseng and Bletillastriata were on the rise as the increasement of the amount of Bletillastriata. The contents of the notoginsengsaponin R1,ginseng saponin Rg1 and ginseng saponin Rb1 of Panax notoginseng single decoction were significantly decreased after compatibility. Therefore,after compatibility,it was more easy to produce（ 20S）-ginseng saponin Rg3 and ginseng saponin Rh4. This study can extend to a method of preparation of（ 20S）-ginseng saponin Rg3 and ginseng saponin Rh4. Furthermore,after compatibility,two ginseng saponins which had lipase inhibitory effect were both increased significantly,indicating that the compatibility of these two herb medicines may have effect on losing weight.

关 键 词：三七 白及 脂肪酶抑制剂 (20S)-人参皂苷Rg3 人参皂苷Rh4 

分 类 号：R285.5[医药卫生—中药学]