黄芩与黄柏协同保护黄药子致肝毒性的实验研究  

Synergistic protective effect of Scutellariae Radix with Phellodendri Chinensis Cortex on Dioscoreae Bulibferae Rhizoma liver toxicity in rats

作　　者：王秋红[1] 杨欣[1] 王蒙[1] 杨炳友[1] 匡海学[1] 

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室黑龙江省中药及天然药物药效物质基础研究重点实验室,黑龙江哈尔滨150040

出　　处：《中国中药杂志》2016年第5期898-903,共6页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2013CB531801);黑龙江中医药大学研究生创新科研项目(20150109);国家自然科学基金面上项目(81473351)

摘　　要：黄药子是临床常用中药之一,临床作用广泛,但是黄药子有毒,容易引起药源性肝损伤。观察黄芩、黄柏及其配伍与黄药子合用时,对黄药子所致肝毒性的缓解作用。该实验采用SD雌性大鼠,黄药子9 g·kg-1连续灌胃28 d造成肝毒性模型,观察各组大鼠肝脏组织学,评估转氨酶及抗氧化酶的活性。形态学和生化指标评估表明,黄药子致大鼠肝毒性模型成功,肝细胞溶解、肿胀、脂肪变性,间隙有炎性细胞,肝组织可见局部点状、片状水肿变性,部分坏死。肝功能相关指标(ALT,AST,ALP)显著升高,具有显著差异(P<0.05或P<0.01)。黄芩、黄柏及其配伍对黄药子引起的肝毒性具有保护作用,其中黄芩配伍黄柏对黄药子引起的肝毒性产生强大的保护作用。能使血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)活性显著降低(P<0.05或P<0.01),肝组织MDA含量显著降低(P<0.001)和GSH含量显著提高(P<0.001),肝组织细胞的病变程度得到明显改善。黄芩和黄柏及其配伍是通过提高肝脏的GSH和抗氧化水平,降低血清ALT,ALP和AST水平,减轻肝组织细胞的损伤,达到保肝的效果。Dioscoreae Bulibferae Rhizoma（ RDB） is commonly used in clinical Chinese medicine. It has been used in many kinds of traditional Chinese medicine（ TCM）,but the toxicity of RDB,easily leads to hepatotoxicity. The objective of the present study is to investigate the synergistic protective effect of Scutellariae Radix（ SR） with Phellodendri Chinensis Cortex（ PCC） on RDB caused liver toxicity in rats. SD female rats were adopted to establish the hepatotoxicity models by RDB（ 9 g·kg-1,ig） once daily for 28 consecutive days. After 28 days,liver histological changes were observed,and the activity of transaminase and antioxidant enzymes was evaluated. Morphological and biochemical indicators evaluation showed that,Dioscoreae Bulibferae Rhizoma-induced hepatotoxicity models were successful,and the liver cells were dissolved and swelling with fatty degeneration; inflammatory cells were present in gaps; local punctiformed or lamellar hydropic degeneration was found in liver tissues,with partial necrosis. Indexes of liver function（ ALT,AST and ALP） were significantly increased（ P〈0. 05 or P〈0. 01）. The combination of SR and PCC has protective effect on RDB-induced hepatotoxicity in rats. SR ＋ PCC exerted the strongest protective effects against RDB-induced hepatotoxicity. SR,PCC,and SB ＋ CP were observed to exhibit hepatoprotective effect as demonstrated by significant decrease in serum levels of alanine aminotransferase（ ALT）,aspartate aminotransferase（ AST）,alkaline phosphatase（ ALP）（ P〈0. 05 or P〈0. 01）,and MAD level in liver tissue（ P〈0. 001）,significant increase in GSH content in liver tissue（ P〈0. 001）,and significant improvement in his to pathologic changes of liver tissues in rats. SR,PCC and their combinations could achieve liver protection effect by reducing ALT,ALP and AST level in serum,increasing GSH level and anti-oxidantability of liver tissues,and reducing hepatic tissue cells injury.

关 键 词：黄芩 黄柏 黄药子 肝毒性 

分 类 号：R285.5[医药卫生—中药学]