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作 者:张平[1,2] 高祥[1] 周毅[1,2] 赵建荣[1] 汪建飞[1,2]
机构地区:[1]安徽科技学院,安徽蚌埠234000 [2]农业部生物有机肥创制重点实验室,安徽蚌埠234000
出 处:《热带作物学报》2016年第4期694-699,共6页Chinese Journal of Tropical Crops
基 金:在滁高校专利发明技术研究项目(No.201308);国家公益性行业(农业)专项经费项目(No.201103004);国家自然科学基金项目(No.31101598);安徽省科技攻关项目(No.1301032151)
摘 要:采用营养液培养的方法,比较Ca_3(PO_4)_2模拟磷胁迫条件时3种氮处理(NO_3^--N、NH_4^+-N、NO_3^--N+NH_4^+-N)对玉米氮素吸收与分配的影响。结果表明,与KH_2PO_4处理相比,磷胁迫处理时,硝态氮、铵态氮及铵硝混合营养处理的玉米整株生物量分别降低38.1%、17.4%和20.8%;与此同时,铵态氮处理的玉米植株全氮、氮素累积量、有机氮及硝态氮含量显著升高,而硝态氮处理时全氮、氮素累积量、有机氮显著降低,硝态氮含量无明显变化;此外,与硝态氮及铵硝混合处理相比,铵态氮处理的玉米叶中可溶性蛋白含量下降幅度最大,而游离氨基酸、脯氨酸含量下降幅度最小。因此,本试验中,铵态氮处理的玉米更耐磷胁迫,表现为增加了氮的吸收和积累以及增强可溶性蛋白降解为氨基酸的过程。A hydroponics experiment was carried out to study the effects of nitrogen forms(ammonium, nitrate and the mixture of both) on the nitrogen uptake and distribution of corn seedling. The experiment was conducted under the condition of phosphorus stress treatment, which was simulated by using Ca3(PO4)2 as the phosphorus form supply. The results showed that, compared with KH2PO4 treatment, the biomass of corn plant under phosphorus stress supplied with ammonium, nitrate and the mixture of both was reduced by 38.1%, 17.4% and 20.8%,respectively. Meanwhile, the content of nitrogen, nitrogen accumulation, nitrate and organic nitrogen were significantly increased in ammonium-treated plants under phosphorus stress, while the supply of nitrate significantly reduced the content of nitrogen, nitrogen accumulation and organic nitrogen, but phosphorus stress had no significant effect on nitrate under nitrate-treated plants. Furthermore, compared with the nitrate and mixed nitrogen treatment,soluble protein content showed the highest reduction rate in the leaves of ammonium-treated plants, however, free amino acids and proline showed the lowest reduction rate in roots, sheaths and leaves, meanwhile, the three had the highest content. Therefore, ammonium treatment rather than nitrate or mixed nitrogen alleviated the negative effects of phosphorus stress resulting of increasing nitrogen uptake and accumulation, and enhancing degradation of protein to amino acid when ammonium was the solely form of nitrogen.
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