高温对豇豆叶片细胞膜脂过氧化和蛋白质表达的影响  

Effects of High Temperature on the Lipid Peroxidation and Protein Expression in Cowpea Leaves

作　　者：崔萌[1] 魏娟娟[1] 苏晓星[1] 邓子贤 潘宇[1] 李金华[1] 苏承刚[1] 张兴国[1] 

机构地区：[1]西南大学园艺园林学院/南方山地园艺学教育部重点实验室,重庆400715

出　　处：《西南大学学报（自然科学版）》2016年第4期21-27,共7页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市重大攻关项目(CSTC2010AA1019)

摘　　要：分析了高温胁迫条件下豇豆[Vigna unguiculata(L.)Walpers]幼苗叶片细胞膜透性和保护酶活性等理化指标的变化及品种间的差异,分析了高温胁迫对豇豆叶片蛋白质表达的影响.结果显示,在高温胁迫下,8个豇豆品种的热害指数差异有统计学意义,直观表现为之豇28-2和白籽豇豆耐热性强,而高产四号、宁豇三号和红豇豆的耐热性差;所有豇豆品种的细胞膜透性和丙二醛(MDA)质量摩尔浓度均有不同程度的增大,但耐热性强的品种增幅较小;超氧化物歧化酶(SOD),过氧化物酶(POD)和过氧化氢酶(CAT)活性呈现出先上升后下降的变化趋势,而耐热性强的品种增幅较大;耐热豇豆品种的可溶性糖浓度及高温胁迫后的增值均低于耐热性较差的品种;双向电泳结果表明,高温胁迫特异性诱导了15种和抑制了6种蛋白质表达,另鉴别到8种上调和10种下调表达蛋白.表明高温胁迫引起一系列生理生化和基因表达的变化.该研究为今后解析豇豆的耐热机理、选育耐热品种和发掘重要抗热基因等奠定了基础.The membrane permeability, protective enzyme activities and other physicochemical indexes in the leaves of cowpea [Vigna unguiculata （L.） Walpers] seedlings were studied, and their intervarietal discrepancies were investigated, while the influence of heat shock on protein synthesis was probed. The re- sults showed that there were significant differentials in heat damage indexes among the eight cowpea varie- ties studied; specifically, Zhijiang 28-2 and White seed were thermotolerant while Gaochan No 4, Ningjiang No 3 and Red cowpea were thermosensitive. Membrane permeability and malondialdehyde （MDA） content increased, in different extents, in all varieties, but the range was narrow between the thermotolerant varieties. SOD, POD and CAT activities of the eight cowpea varieties increased at first and then decreased; the content and augment of the soluble sugar of thermosensitive varieties were higher than those of the thermotolerant ones. Employing PDQuest 8.0 software to analyze 2-DE gels, we detected that 15 novel protein spots appeared, 6 spots disappeared, 8 spots were up-regulated and 10 spots were down- regulated on the sample gel of 45 ℃ 5 h treatment. The results indicated that fundamental physiology and biochemistry and the gene expression were changed under high temperature stress.

关 键 词：豇豆 高温胁迫 膜脂过氧化 防御酶 蛋白质表达 

分 类 号：S330[农业科学—作物遗传育种]