PCR技术检测鲤鱼组织吉陶单极虫DNA  

Detection of Thelohanellus Kitauei in Cyprinus Carpio by PCR

作　　者：冯现维[1] 杨振国[1] 闫广谋[1] 周玉[1] 穆占昆[1] 徐广宏 张鹏[3] 

机构地区：[1]解放军军需大学,吉林长春130062 [2]辽宁省辽中县水产技术推广站 [3]辽宁省铁岭市水产技术推广站

出　　处：《动物医学进展》2002年第5期62-63,共2页Progress In Veterinary Medicine

基　　金：国家自然科学基金资助 (39870 0 5 4)

摘　　要：采用 PCR方法对鲤鱼的鳃、口腔黏膜、肌肉、心、肝、脾、肾、肠等组织进行了吉陶单极虫 DNA的检测。从患病及健康鲤鱼的上述组织提取模板 DNA,用吉陶单极虫的特异引物进行 PCR扩增。结果 :在发病池 3条患病鲤鱼肠道均扩增出一条长度为 560 bp左右的特异片断 ,而其他组织及其他鲤鱼各组织均未检测到吉陶单极虫 DNA。初步认为吉陶单极虫在鲤鱼体内仅寄生在肠道。PCR was used to detect Thelohanellus kitauei from viscera in Cyprinus carpio. Template DNA were extracted from viscera organisms and intestinal parasite, while special primers were used to amplify. DNA of Thelohanellus kitauei was founded in intestines organisms of all 3 diseased carps, but was not found in other organisms and other carps. The study suggested that Thelohanellus kitauei only live in intestine organisms as a parasite.

关 键 词：PCR技术 检测 鲤鱼组织 吉陶单极虫DNA 

分 类 号：S941.513[农业科学—水产养殖]