马急性滑膜炎模型关节液中MMP-3和MMP-13含量变化的研究  

Changes in synovial fluid MMP-3 and MMP-13 from horses with experimentally-induced synovitis

作　　者：王冠颖[1] 姜仁礼 李欣然[1] 范晓静[1] 李玥[1] 刘旭[1] 王晶璐 潘佳亮[1] 陈希[1] 马云峰[1] 赵洋[1] 魏成威[1] 韩光[1] 高利[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2016年第2期28-30,共3页Chinese Journal of Veterinary Medicine

基　　金：国家科技支撑计划资助(2012BAD46B02-03)

摘　　要：关节滑膜炎是骨关节疾病发病的基本病理过程,试验通过研究马急性滑膜炎关节液中基质金属蛋白酶-3(MMP-3)和基质金属蛋白酶-13(MMP-13)含量的变化,进而研究马滑膜炎机理。试验选用8匹马,在马右侧中间腕关节内注入0.5 ng大肠杆菌脂多糖(LPS)诱导滑膜炎,同时在对侧关节内注入等量的PBS作为对照,于注射药物前和注射药物后的8、24 h和168 h,采马关节液.用ELISA检测马关节液中MMP-3和MMP-13的含量。结果注入LPS的关节液中MMP-3和MMP-13的量在注射后的24 h内持续升高,在24 h达到最大量10.01 ng/mL和6.08 ng/mL,与对照组比较差异性显著,24 h后开始下降,168 h时含量恢复到对照水平。试验结果表明,12'S诱导的滑膜炎模型为一过性的.在急性滑膜炎过程中,MMP-3和MMP-13的表达和释放增加,并且这两种酶在滑膜炎过程中起到重要作用。To study the synovitis mechanism of the horse,changes in synovial fluid MMP-3 and MMP-13 from horses with acute synovitis were analyzed.Acute synovitis was induced in 8 skeletally mature mares by a sterile intra-articular injection of O.S ng lipopolysaccharide（LPS）into the right middle carpal joint.The same doge sterile PBS was injected into the left control joint.Synovial fluid was collected at post injection hours（PIH） 0,8,24 and 168.Concentrations of MMP-3 and MMP-13 were measured using ELISA kit.Our resits showed that MMP-3 and MMP-13 increased steadily through PIH 24,and peaked at PIH 24 with the amount of 10.01 ng/mL and 6.08 ng/mL.The differences were significantly when compared with control group MMP-3 and MMP-13 returned to baseline by PIH 168.The results indicate injections of LPS into equine carpal joints resultes in a transient inflammatory response,and the expression and release of MMP-3 and MMP-13 are increased in acute synovitis.

关 键 词：滑膜炎 脂多糖 基质金属蛋白酶-3 基质金属蛋白酶-13 

分 类 号：S857.165[农业科学—临床兽医学]