microRNA-491-5p血浆水平及其靶基因多态性与早发冠心病易感性的研究  

Relationship of plasma levels of microRNA 491-5p and its target gene polymorphism with the prognosis of patients with premature coronary artery disease

作　　者：张岩[1] 王聪霞[1] 马维冬 李永勤[1] 韩振华[1] 张春艳[1] 吴皓宇[2] 权晓慧[3] 惠静[1] 

机构地区：[1]西安交通大学第二附属医院心血管内科,陕西西安710004 [2]陕西省人民医院心血管内科,陕西西安710068 [3]西安市第一医院心血管内科,陕西西安710002

出　　处：《西安交通大学学报（医学版）》2016年第3期318-321,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81273878)~~

摘　　要：目的通过病例对照研究,探讨陕西省汉族人群血浆miRNA-491-5p水平和miRNA-491-5p(has-miR-491-5p)靶基因基质金属蛋白酶-9(MMP-9)单核苷酸多态性(SNP)改变与早发冠心病(pCAD)发生风险及预后的关系。方法连续收集pCAD病例270例,对照组300例。用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)检测has-miR-491-5p靶基因MMP-9rs1056628的多态性,同时比较各种基因型与pCAD发生的相关性。结果 rs1056628位点存在A-C多态性,与CC基因型(发生率42%)相比,携带CA、AA基因型的个体冠心病的发生率为31%,差异有统计学意义(P=0.045);携带CA/AA基因型冠心病在低水平总胆固醇(TC)、较低水平的低密度脂蛋白胆固醇(LDL-C)人群中的患病风险降低更为显著。结论 has-miRNA-491-5p靶基因MMP-9rs1056628C-A多态性改变可降低pCAD的发生率,携带C等位基因是pCAD发病的独立危险因素。Objective To study the relationship of the plasma levels of miRNA-491-Sp in Han population in Shaanxi Province and the changes of single nucleotide polymorphisms （SNP） of the target gene matrix metalloproteinases-9 （MMP-9） of miRNA-491-5p （has-miR-491-5p） with the incidence risk and prognosis of premature coronary artery disease （pCAD） through the case-control design. Methods In this study, we made a consecutive recruitment of 270 pCAD cases in the case group and 300 cases in the control group. Using the polymorphism method of polymerase chain reaction and restriction fragment length （PCR-RFLP）, target gene MMP-9 of has-miR-491-5p and rs1056628 genotypes was detected to compare the association between the variant genotypes and pCAD. Results In the changes of rs1056628C-A polymorphisms, compared with that of CC genotypes （the incidence was 42%）, the risks of having coronary heart disease in the individuals carrying CA and AA genotypes were 31%, the difference was statistically significant （P = 0. 045）. The risks of developing coronary heart disease in the individuals carrying CA and AA genotypes were reduced more significantly in the population with low total cholesterol （TC）, and low low-density lipoprotein cholesterol （LDL-C）. Conclusion Target gene MMP-9 of has-miRNA-491-5p rs1056628C-A polymorphism is associated with the reduced incidence risk of pCAD, and carrying C alleles is an independent risk factor for pCAD.

关 键 词：早发冠状动脉性心脏病 微小RNAS 单核苷酸多态性 基质金属蛋白酶-9(MMP-9) miRNA-491-5p(has-miR-491-5p) 

分 类 号：R541.4[医药卫生—心血管疾病]