一枝蒿黄酮类提取物体内抗乙肝病毒活性及化学成分研究  

Study on in vivo anti-hepatitis B virus activity and chemical constituents analysis of flavonoids fromArtemisia rupestris L.

作　　者：杨璐[1,2] 张素挽 刘中奇[3] 贺金华 戎晓娟 顾政一 

机构地区：[1]新疆医科大学药学院药剂物化教研室,乌鲁木齐830011 [2]新疆维吾尔自治区药物研究所,乌鲁木齐830004 [3]解放军第四军医大学,西安710000

出　　处：《新疆医科大学学报》2016年第5期578-581,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金新疆联合基金(U1303224)

摘　　要：目的研究一枝蒿黄酮类提取物抗鸭乙型肝炎病毒(DHBV)作用及化学成分。方法一枝蒿药材经50%乙醇提取,采用聚酰胺柱进行分离纯化,得到一枝蒿30%、50%乙醇洗脱物。采用1日龄北京鸭,人工感染鸭乙型肝炎病毒模型,将阳性鸭随机分为30%乙醇洗脱物组、50%乙醇洗脱物组、阿德福韦酯组、病毒对照组,各组分别于给药前、给药期间不同时间(5d和10d)和停药后3d取血,分离血清;停药后第3天杀剖,取肝脏,以血清DHBV-抗原、血清DHBV-DNA、肝脏DHBV-DNA作为药效评价指标,研究其体内抗乙肝病毒的作用。采用LCHRMS/MS法对有效部位化学成分进行分析。结果 50%乙醇洗脱物在200mg/kg剂量下能显著抑制鸭乙肝病毒血清和肝脏中DNA复制,30%乙醇洗脱物的抑制作用较弱。从50%乙醇洗脱物中推测出5种主要化合物,分别为6-去甲氧基-4′-O-甲基茵陈色原酮-7-O-β-D-葡萄糖苷、紫花牡荆素、异山奈甲黄素、洋艾素、山柰素-3,3′,4′-三甲醚。结论 50%乙醇洗脱物有抗HBV的作用,50%乙醇洗脱物中推测出的5种主要化合物可能在抗HBV中起到重要的作用。Objective To study the anti-DHBV effect and chemical constituents of flavonoids extract from Artemisia rupestris.Methods The separation and purification of the 50% ethanol extract of Artemisia rupestris L.were performed with polyamide column.30%and 50%ethanol-eluted fractions were obtained.1-day old Beijing ducklings were used to establish the model of duck Hepatitis B Virus.The positive ducks were randomly divided into 30% ethanol extract group,50% ethanol extract group,adefovir dipivoxil group and virus control group.The blood of ducks were collected and the serum were isolated before medication,and at the 5th,10 th day after medication,and the 3rd day after stopping medication;These ducks were killed and livers were removed on the third day after stopping medication.The activity of in vivo antiHBV was determined by Serum DHBV antigen,Serum DHBV-DNA and Liver DHBV-DNA detection with the method of immunohistochemistry.The ethanol-eluted fractions and the components were analyzed by LC-HRMS/MS.Results 50% ethanol-eluted fraction can significantly inhibit DHBV-DNA replication in serum and liver in the 200mg/kg dose.30% ethanol-eluted fraction has weak inhibition.Five compounds were gained from 50% ethanol-eluted fraction as 6-demethoxy-4′-O-methylcapillarisin-7-O-β-D-glucopyranoside,Caticin,kaempferide,artemetin,kaempferol 3,3′,4′-trimethylether.Conclusion 50% ethanol-eluted fraction can significantly inhibit DHBV-DNA replication in serum and liver,indicating that 50%ethanol-eluted fraction has an effect on anti-HBV.

关 键 词：一枝蒿 黄酮类 鸭乙型肝炎病毒(DHBV) 化学成分 

分 类 号：R966[医药卫生—药理学]