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机构地区:[1]宁波城市职业技术学院,浙江宁波315100 [2]温州医科大学健康与环境生态研究所,浙江温州325035 [3]宁波市森林病虫防治检疫站,浙江宁波315100
出 处:《Agricultural Science & Technology》2016年第4期920-922,950,共4页农业科学与技术(英文版)
基 金:Ningbo Natural Science Foundation(Grant No.201201A6104001);Zhejiang Province Department of Education Research Projects(Grant No.ZWT14048)~~
摘 要:[Objective] The aim is to identify the composition of female sex pheromones of Orthaga achatina and to understand the differential attractiveness of these components to Orthaga achatina moths. [Method] The pheromone glands of female moths were extracted and analyzed using gas chromatography coupled with mass spectroscopy(GC-MS); the chemical analysis was confirmed by electroantennogram(EAG) bioassays; a field trial was conducted to confirm the optimized blends. [Result] Z11-16:Ac could elicit the largest EAG responses in male moths at all the doses tested. EAG responses to plant volatiles and to the pheromone isomers of Z11-16:Ac were at a similar level and lower than the responses to Z11-16:Ac. In the field tests, it was demonstrated that synthetic Z11-16:Ac was significantly more attractive to male O. achatina moths, that its pheromone isomers, whether these were presented alone or in mixtures with Z11-16:Ac. [Conclusion](Z)-11-hexadecenyl acetate(Z11-16:Ac) was identified as the main component of the sex pheromone and it was demonstrated that synthetic Z11-16:Ac was significantly more attractive to male O. achatina moths.[目的]研究樟巢螟雌蛾性信息素的主要组分及其诱蛾活性。[方法]利用气质联用仪(GC-MS)对樟巢螟雌蛾的性腺体提取物和合成标样比较分析,并用与樟巢螟雌蛾性信息素主要组分的异构体进行触角点位的生理测定,最后开展田间诱集比较试验以筛选出最佳性信息素混合物。[结果]雄虫触角对各种剂量的Z11-16:Ac产生强烈的EAG反应。在同一水平下,樟巢螟雄虫触角对植物气味物质和Z11-16:Ac异构物的EAG反应都低于Z11-16:Ac。在田间试验中,单一的Z11-16:Ac或其混合物质均能诱集樟巢螟雌虫。[结论]Z11-16:Ac是樟巢螟雌蛾性信息主要组分,对樟巢螟雄蛾具有诱集活性。
关 键 词:Orthaga achatina Sex pheromone ELECTROANTENNOGRAM Field bioassay
分 类 号:S433[农业科学—农业昆虫与害虫防治]
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