自拟平脉定悸方对快速性心律失常模型大鼠血清HSP70、NF-κB影响  

Influence from Self-made Pingmai Dingji Decoction to Heat Shock Protein 70 and Nuclear Factor κB in Serums of Rats with Tachycardiac Arrhythmia

作　　者：高业[1] 昌艳艳[2] 蔺瑶峰 张高峰[1] 李重阳[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《辽宁中医药大学学报》2016年第5期22-24,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁省科学计划项目(2011225040)

摘　　要：目的:探讨自拟平脉定悸方对快速性心律失常大鼠血清中热休克蛋白70、核因子κB的影响。方法:70只健康雄性SD大鼠随机选取12只作为空白对照组A组,其余58只大鼠予以激惹情志诱导刺激,每天监测大鼠心电图,两周后将造模成功的大鼠随机选出48只,随机分为4组。B组模型组予生理盐水(2 m L/只)灌胃,C组予平脉定悸方(100 m L/kg)灌胃,D组给予酒石酸美托洛尔片(0.1 mg/kg)灌胃,E组同时给予平脉定悸方(100 m L/kg)和酒石酸美托洛尔(0.1 mg/kg)灌胃,连续14 d,每日1次,采血。应用ELISA法检测血清中热休克蛋白(HSP70)和核因子κB(NF-κB)的含量。结果:(1)C、D、E组血清HSP70含量明显高于造模前对照A组(P<0.05)。(2)C、E两组均较B组血清HSP70含量明显升高(P<0.05)。(3)B组较A组血清HSP70含量升高不明显(P>0.05)。(4)D组较B组血清HSP70升高不明显(P>0.05)。(5)C、D两组较A组血清NF-κB含量明显升高(P<0.05)。(6)B、E两组较之A组血清NF-κB升高不明显(P>0.05)。(7)D组较C、E两组血清NF-κB升高明显(P<0.05)。结论:自拟平脉定悸方通过促进HSP70和抑制NF-κB的激活和表达治疗快速性心律失常。提示平脉定悸方在治疗快速性心律失常上具有多途径、多层面的治疗特点,具有西药无法比拟的优势。Objective：To explore the influence from Self-made Pingmai Dingji Decoction to heat shock protein 70 and nuclear factor κB in serums of rats with tachycardiac arrhythmia. Methods：We randomly chose 12 rats as blank control group A from 70 healthy male SD rats,and the rest was induced to produce stimulation by irritating emotion. We monitored electrocardiogram of the rest rats everyday,and chose 48 rats from rats which were built model successfully two weeks later. They were randomly divided into four groups. Model group B was given 2 m L physiological saline every rat,group C was given Self-made Pingmai Dingji Decoction by lavage in the dose of 100 m L/kg,group D was given metoprolol tartrate tablets by lavage in the dose of 0.1 mg/kg,while group E was given Self-made Pingmai Dingji Decoction（100 m L/kg）and metoprolol tartrate tablets（0.1 mg/kg）by lavage. The feeding process was last for 14 consecutive days and the blood sampling of all rats was at a frequency of once a day for all groups. In the end,we used the ELISA method to detect the content of heat shock protein（HSP70）and nuclear factor of κB（NF-κB）in serums. Results：（1）The contents of heat shock protein（HSP70）in serums of group C,group D and group E were significantly higher than that of control group A before building model（P〈0.05）.（2）Group C and group E were both significantly higher than group B in the contents of HSP70 in serums（P〈0.05）.（3）The elevated degree of HSP70 in serums of group B was not obvious than that of group A（P〉0.05）.（4）The elevated degree of HSP70 in serums of group D was not obvious than that of group B（P〉0.05）.（5）The contents of NF-κB in serums of group C and group D were significantly higher than that of control group A（P〈0.05）.（6）The elevated degree of NF-κB in serums of group B and group E was not obvious than that of group A（P〉0.05）.（7）Group D was significantly higher than group C and group E in the contents of NF-κB in serums（P〈0.05

关 键 词：心律失常 热休克蛋白70 核因子ΚB 大鼠 

分 类 号：R541.71[医药卫生—心血管疾病]