滋阴基础上清热化痰与益气活血序贯治疗IPF实验研究  

Experimental Study of Sequential Treatment of IPF by Clearing Heat and Resolving Phlegm,Supplementing Qi and Activating Blood on the Basis of Nourishing Yin

作　　者：吴谦[1] 李丽君[1] 耿青霞[1] 葛东宇[1] 李根茂[1] 王淑艳[1] 李丽娜[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《辽宁中医药大学学报》2016年第5期39-42,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：北京中医药大学自主选题项目(2014-JYBZZ-XS-012)

摘　　要：目的：观察滋阴基础上的清热化痰与益气活血序贯疗法对IPF小鼠肺组织形态学,血清中IL-6、TGF-β含量的影响。方法：SPF级ICR雄性小鼠105只,体重16~20 g,随机分为正常对照组、模型组、西药治疗组以及4个中药治疗组,每组15只,除正常对照组外,其余各组采用气管内注射博莱霉素（5 mg/kg）复制小鼠肺纤维化模型。造模第2天起,正常对照组和模型组用生理盐水灌胃,西药组按0.15 mg/g给予甲基泼尼松。4个中药治疗组分别按照不同阶段不同药物不同时限给药,于21 d处死小鼠。HE染色及武兆发简化Mallory氏胶原染色,观察肺组织形态变化;双抗夹心酶联免疫吸附试验（ELISA）法检测血清中IL-6、TGF-β的含量。结果：HE染色及胶原染色显示,模型组小鼠支气管周围及肺泡间隔有大量炎症细胞浸润并伴有出血性渗出,纤维化程度明显。西药组较模型组虽好转但效果不及中药组明显。中药12组肺泡炎明显好转,肺泡内未见明显的炎性细胞,肺泡隔、细小支气管周围的纤维化病灶较模型组有明显改善,肺泡结构已基本恢复。模型组小鼠血清中IL-6、TGF-β含量均高于正常组,与模型组比较,中药12组小鼠血清中IL-6、TGF-β含量降低显著。结论：从以上各项实验指标观察来看,中药12组能明显控制与改善肺纤维化,降低炎症细胞因子IL-6、TGF-β的含量,提示清热化痰药适宜在肺纤维化早期使用,不宜贯穿使用于整个病程,益气活血药可在病程中后期使用。Objective：To observe the effect of sequential therapy by clearing heat and resolving phlegm,supplementing Qi and activating blood on the basis of nourishing Yin for the lung morphology and the IL-6,TGF-β content in serum expression level in mice with IPF morphology. Methods：105 SPF male ICR mice,weighed 16~20 g,were randomly divided into normal control group,model group,western medicine group,and 4 traditional Chinese medicine treatment group,with 15 rats in each group,except the normal control group,other groups duplicated the model of pulmonary fibrosis in mice by intratracheal injection of bleomycin（5 mg/kg）. After the second day of modeling,normal control group and model group were administrated with normal saline,western medicine group was given by 0.15 mg/g methylprednisolone. 4 Chinese medicine treatment groups respectively were administered in different time according to the different stages of different drugs,the mice were sacrificed on the 21 th day. By HE staining and simplified WU Zhaofa Mallory’s collagen staining,morphological changes of lung tissue were observed;by enzyme-linked immuno sorbent assay（ELISA） method,IL-6,TGF-β in serum were detected. Results：HE staining and collagen staining showed that the mice in the model group,bronchial and alveolar septum were rounded a large number of inflammatory cell infiltration with hemorrhage exudation and significant fibrosis. The western medicine group turned better than the control group obviously,but the effect was less than the traditional Chinese medicine group. Alveolitis in traditional Chinese medicine 1 2 groups turned better markedly,there were no obvious inflammatory cells in alveolar septum,small peribronchial fibrosis lesions were significantly improved compared with the model group,the alveolar structure had been basically restored. IL-6,TGF- beta content in serum of mouse model group were higher than the normal group,compared with model group,IL-6,TGF-β content in serum of Chinese medicine 12 groups mice were decreased sign

关 键 词：特发性肺纤维化 序贯疗法 滋阴 清热化痰 益气活血 

分 类 号：R563[医药卫生—呼吸系统]