丹参黄芪鲫鱼药膳汤预防大鼠早期动脉粥样硬化实验研究  

Experimental Study of the Preventive Effect of the Danshen Huangqi Jiyu Decoction on Early Atherosclerosis Rats

作　　者：唐妮[1] 高静[1] 吴晨曦[1] 叶艳[1] 吴霜[1] 朱蓉[1] 

机构地区：[1]成都中医药大学,四川成都611137

出　　处：《辽宁中医药大学学报》2016年第5期45-49,共5页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的:探讨丹参黄芪鲫鱼药膳汤预防大鼠早期动脉粥样硬化(AS)的作用。方法:采用高脂饲料+腹腔注射Vit D3建立AS动物模型。将72只大鼠随机分为空白组、模型组、阳性对照组、丹参黄芪鲫鱼药膳汤组(简称药膳方组)、丹参黄芪汤拆方组、鲫鱼汤拆方组共6组,每组12只;在造模同时给予干预:药膳方组灌喂丹参黄芪鲫鱼汤,丹参黄芪汤拆方组灌喂丹参黄芪汤,鲫鱼汤拆方组灌喂鲫鱼汤,阳性对照组灌喂阿托伐他汀钙溶液,空白组与模型组灌喂生理盐水,1次/d,共干预12周。期间每日观察大鼠一般情况并测体质量,于第12周末采血后处死大鼠,测定血清超敏C反应蛋白(hs-CRP),HE染色光镜下观察胸主动脉和肝脏病理组织变化。结果:共7只大鼠未完成实验,其中药膳方组、丹参黄芪汤拆方组和鲫鱼汤拆方组各死亡大鼠1只,模型组和阳性对照组各死亡2只。成功建立了大鼠早期AS模型:模型组大鼠hs-CRP明显升高,与空白组比较,差异有统计学意义(P<0.05);胸主动脉中膜弹性纤维排列紊乱、疏松,内皮细胞部分缺失,内膜下大量泡沫细胞聚集,可见炎细胞浸润等,肝脏出现大泡样脂肪变性,炎细胞浸润,肝窦扩张充血,出现局部性坏死及少许纤维组织增生,均提示形成早期AS。药膳方组体质量均高于模型组及丹参黄芪汤拆方组,差异有统计学意义(P<0.05);药膳方组hs-CRP低于模型组,差异有统计学意义(P<0.05)。药膳方组主动脉病变及肝脏病变较轻,其病理改变明显轻于模型组。结论:丹参黄芪鲫鱼药膳汤可通过降低大鼠早期动脉粥样硬化发生的炎症反应达到抗炎作用,以预防早期AS的发生发展。Objective：To explore the preventive effect of the Danshen Huangqi Jiyu Decoction（DHJD）on early atherosclerosis rats. Methods：Using atherogenic diet and Vitamin D3 to establish a rat model of atherosclerosis. Seventy-two male wistar rats were randomly divided into 6 groups：the control group,model group,positive control group,Danshen Huangqi Jiyu Decoction（DHJD）group,Danshen Huangqi Seperated Prescription group and Jiyu Seperated Prescription group,12 rats in each group. Intervention was given while modeling：the DHJD group were fed with the Danshen Huangqi Jiyu Decoction,the Danshen Huangqi Seperated Prescription group were fed with Danshen Huangqi Decoction,the Jiyu Seperated Prescription group were fed with Jiyu Decoction,the positive control group were fed with atorvastatin,the control group and model group were fed with normal saline,once daily. The experiment lasted 12 weeks. During the time,observed rats in general condition and weighed them every day. The rats were killed at the end of the 12 th week,determining the serum hypersensitive c-reactive protein（hs-CRP）and to investigate the structural changes of thoracic aorta and liver. Results：Seven rats died during the study,among them,the DHJD group,Danshen Huangqi Seperated Prescription group and Jiyu Seperated Prescription group each has one death,the model group,positive control group each has two death. Succeed in establishing the modle of early atherosclerosis：comparing with the control group,the hs-CRP of the model group is increased significantly,the difference was statistically significant（P〈0.05）. Elastic fiber of the thoracic aorta was arranged at random and loose,absence of the endothelium,foam cells accumulated in the intima and inflammatory cell infiltration,etc. Hepatic tissue had fatty degeneration,inflammation cells,tissue death,hyperplasia of fibrous tissue and hepatic sinusoid extended with hyperemia. The above suggested the model of early was formed. The weight of the DHJD group was higher than each of the model

关 键 词：药膳 动脉粥样硬化 超敏C反应蛋白 炎症反应 动物实验 

分 类 号：R543.5[医药卫生—心血管疾病]