非洲木犀榄叶中木犀草苷、橄榄苦苷提取工艺考察  

Optimum Extraction Process of Luteoloside and Oleuropein in Olea Europaea Subsp. Africana

作　　者：王和宇[1,2] 马学盛 李春娜[1,4] James Campbell 秦玲玲[1] 张晶[2] 徐暾海[1,4] 刘铜华[1] 

机构地区：[1]北京中医药大学中医养生学教育部重点实验室,北京100029 [2]吉林农业大学中药材学院,吉林长春130118 [3]西开普大学 [4]北京中医药大学中药学院,北京100102

出　　处：《辽宁中医药大学学报》2016年第5期72-74,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家国际科技合作专项项目(2012DFG31550);北京中医药大学创新团队项目(2011-CXTD-19)

摘　　要：目的:考察非洲木犀榄叶中木犀草苷、橄榄苦苷的最佳提取工艺。方法:采用HPLC法测定非洲木犀榄叶中木犀草苷、橄榄苦苷的含量,使用超声波提取法进行提取,考察料液比、溶剂分数、提取时间、超声功率对含量的影响。在单因素的基础上,结合正交试验法选取溶剂分数、料液比、提取时间考察其对提取工艺的影响,确定最佳提取工艺。结果:非洲木犀榄叶中木犀草苷、橄榄苦苷的最适提取工艺为70%甲醇溶液,料液比为1∶40,超声功率400 W,提取时间30 min。结论:该方法对非洲木犀榄叶中木犀草苷、橄榄苦苷的提取简单易行,方法可靠。Objective：To study the optimized method of extraction luteoloside and oleuropein from Olea europaea L. subsp. Africana. Methods：The contents of luteoloside and oleuropein in Olea europaea L. subsp. Africana were measured using HPLC method. The method of extraction luteoloside and oleuropein from olive was optimized with the assistant of ultrasonic. Four factors below were tested according to the extraction yield：the ratio of material to solvent,methyl alcohol concentration,extraction time and ultrasonic power,the optimum extraction conditions of luteoloside and oleuropein in Olea europaea L. subsp. Africana were determined with ort-hogonal test. Results：The optimum conditions of extracting were obtained as follow：70% methyl alcohol as extraction solvent at 400 W of Ultrasonic energy for 30 min,and the radio of solvent to material was 1∶40. Conclusion：The method of extraction luteoloside and oleuropein from Olea europaeasubsp. Africana is simple and good.

关 键 词：非洲木犀榄 高效液相色谱法 木犀草苷 橄榄苦苷 超声波提取法 正交试验 提取工艺考察 

分 类 号：R284[医药卫生—中药学]