芍药苷醇质体含量测定及包封率研究  

Determination of Content and Entrapment Efficiency of Paeoniflorin Ethosomess

作　　者：袁杰[1] 张铭[1] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《辽宁中医药大学学报》2016年第5期87-89,共3页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的：建立芍药苷醇质体含量及包封率的测定方法。方法：采用注入法制备醇质体,以超速离心法分离醇质体和游离药物,建立HPLC测定芍药苷含量的方法。结果：芍药苷浓度在0.966～4.8μg范围内与峰面积呈良好的线性关系（r=0.9997）,回收率为97.6%～100.8%,日内及日间精密度均小于1.5%,样品8 h内稳定性良好,平均包封率为81.03%。结论：该方法准确可靠方便快捷,可用于芍药苷醇质体中药物含量及包封率的测定。Objective：To establish a method for the determination of content and entrapment efficiency of paeoniflorin ethosomes. Methods：Paeoniflorin ethosomes was prepared with ETOH injection method. Ultracentrifugation method was employed to separate the free drug from the ethosomes. The content of Paeoniflorin was determined by an HPLC method. Results：A good linear relationship was found between the peak area and the concentration of Paeoniflorin ranging from 0.966～4.8 μg（r=0. 9997）. The recovery of paeoniflorin was in a range of 97.6%. Intra-day and inter-day RSDs were less than 1.5%. Paeoniflorin was stable in theprepared samples for 8 h at ambient temperature. The freedrug was well separated from the ethosomes by ultracentrifugation method. The average entrapment efficiency of paeoniflorin in ethosomes was 81.03%. Conclusion：The method is simple,accurate andsuitable for determination of content and entrapment efficiency of paeoniflorin ethosomes.

关 键 词：芍药苷 醇质体 包封率 

分 类 号：R284[医药卫生—中药学]