中国莱姆病螺旋体菌株bmpA基因中人B细胞表位多态性分析  

Polymorphisms of human B cell epitopes in bmpAof Borrelia burgdorferi sensu lato in China

作　　者：刘慧鑫[1] 郝琴[2] 刘炜[2] 侯学霞[2] 张琳[2] 万康林[2] 

机构地区：[1]温州医科大学检验医学院生命科学学院,温州325035 [2]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京102206

出　　处：《中国人兽共患病学报》2016年第4期321-326,共6页Chinese Journal of Zoonoses

基　　金：国家科技重大专项课题(No.2013ZX10004-001)资助~~

摘　　要：目的研究我国伯氏疏螺旋体(Borrelia burgdorferi sensu lato),即莱姆病螺旋体菌株中bmpA基因及其人B细胞表位区的序列多态性。方法选取61株莱姆病螺旋体分离株,PCR扩增其bmpA基因并测序后,结合4种基因型参考菌株序列,与从免疫表位数据库(Immune Epitope Database,IEDB)中查询到的B细胞表位序列进行比对分析。结果中国B.garinii基因型菌株的cluster2、cluster3以及B.afzelii和B.valaisiana基因型所有菌株与参考序列比对后发现分别有3、9、5和18个异义突变。B细胞表位改变主要发生在B.afzelii基因型全部分离株和B.garinii基因型的cluster3及5株非成簇菌株。B.afzelii基因型菌株在B细胞表位区的异义突变A125S影响了2个B细胞表位;B.garinii基因型的cluster3和5株非成簇菌株B细胞表位区的3个异义突变导致5个B细胞表位均发生改变。结论中国莱姆病螺旋体bmpA基因在4种基因型间和B.garinii基因型内存在较大多态性。To study the polymorphism in bmpAand its human B cell epitopes of Borrelia burgdorferi strains in China,the bmpAsequences of 61 B.burgdorferi strains were obtained by PCR and sequencing.They were compared with the human B cell epitope sequences from Immune Epitope Database（IEDB）based on the reference strain of each genotype.The 3,9,5and18 nonsynonymous mutations were seperately found in cluster2 and cluster3of B.garinii,B.afzelii and B.valaisianastrains in China.The changed B cell epitopes were mainly found in cluster3 and five non-clustered strains of B.garinii and B.afzelii strains.The nonsynonymous mutation of A125 Sin B.afzelii strains affected two B cell epitopes.Three nonsynonymous mutations in cluster3 and five non-clustered strains in B.garinii affected all of five B cell epitopes.The polymorphysims were widely existed in bmpAand its B cell epitopes in B.garinii and between four genotypes of B.burgdorferi strains selected in China.

关 键 词：莱姆病螺旋体 bmpA B细胞表位 多态性 

分 类 号：R377[医药卫生—病原生物学]