壮骨方对糖尿病大鼠骨质疏松的防治及其对血清IGF-1、TNF-α水平的影响  

The effect of Zhuanggu-decoction on the treatment and on serum IGF-1 and TNF-α in type 2 diabetes rats with osteoporosis

作　　者：粟麟 李双蕾[2] 陈文辉[2] 马汝洁 莫文秋 

机构地区：[1]广西中医药大学研究生院,南宁530001 [2]广西中医药大学第一附属医院,南宁530001 [3]要江苏连云港市赣榆区中医医院,连云港222100

出　　处：《中国骨质疏松杂志》2016年第4期428-432,共5页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(81160486);广西名老中医民族医传承工作室建设项目(桂卫中[2014]9号文件)

摘　　要：目的探讨壮骨方对糖尿病大鼠骨质疏松进程的干预作用及其对血清IGF-1、TNF-α水平的影响。方法建立2型糖尿病大鼠模型,分为空白组、模型组、二甲双胍组及壮骨方高、中、低剂量6组,分别给予相应药物灌胃12周。12周后测定各组大鼠全身骨密度,行胫骨干骺端病理切片测定骨计量参数,采用酶联免疫吸附法测定各组血清IGF-1、TNF-α的水平。结果模型组大鼠的骨密度、骨剂量参数、血清IGF-1水平均明显下降,血清TNF-α表达增高(P<0.01),壮骨方可提高血清IGF-1、降低TNF-α水平,防止骨密度、骨参数下降(P<0.01),且中药高剂量组作用均优于二甲双胍组(P<0.01)。结论壮骨方可能通过升高血清IGF-1水平、降低TNF-α水平,进而影响糖尿病骨代谢进程。Objective To study the effect of the traditional Chinese medicine,Zhuanggu-decoction,on serum IGF-1 and TNF-αin type 2 diabetes rats during the process of osteoporosis. Methods Rat type 2 diabetic model was established and the rats were divided into 6 groups： blank group,DMgroup,DM＋ metformin group（ MET）,DM＋ MET ＋ lowdose Zhuanggu-decoction（ ZGF-L） group,DM＋ MET ＋ medium dose Zhuanggu-decoction（ ZGF-M） group,and DM＋ MET ＋ high dose Zhuanggudecoction（ ZGF-H） group. The rats were gavaged with corresponding drugs for 12 weeks. After 12 weeks,the bone mineral density,quantitative bone histomorphometry of the tibial stem epiphyseal,and serum IGF-1 and TNF-α were measured in each group. Results The bone mineral density,quantitative bone histomorphometry parameters,and serum IGF-1 decreased,but serum TNF-α increased in DMgroup（ P〈0. 01）. Serum IGF-1 increased,serum TNF-α decreased,and the decrease of bone mineral density and histomorphometry parameters was prevented in Zhuanggu-decoction groups. The effect in DM＋ MET ＋ ZGF-H group was better than that in DM＋ MET group（ P〈0. 01）. Conclusion Zhuanggu-decoction affects osteoporosis process by increasing serum IGF-1 and decreasing serum TNF-α level in type 2 diabetic rats.

关 键 词：中医中药 壮骨方 IGF-1 TNF-Α 2型糖尿病骨质疏松症 

分 类 号：R587.2[医药卫生—内分泌]