淋巴毒素α基因rs2239704 A>C位点多态性与川崎病的关联性研究  

Association of locus rs2239704 A>C polymorphisms of lymphotoxin-alpha gene with Kawasaki disease

作　　者：曾才秀 詹霞[2] 周进 谢圭 袁海斌 周钗 杨作成[2] 

机构地区：[1]湖南省湘潭市妇幼保健院儿科,411100 [2]中南大学湘雅三医院儿科,长沙410013

出　　处：《中华临床医师杂志（电子版）》2016年第8期26-29,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：湘潭市科技局科研基金资助(SF20142002)

摘　　要：目的探讨淋巴毒素α（LTA）基因rs2239704 A〉C位点多态性与儿童川崎病（KD）及其并发冠状动脉损伤（CAL）的关联性。方法收集2000至2015年间100例川崎病病例与92例健康儿童血样本,提取外周血基因组DNA,应用基因测序方法检测LTA基因rs2239704 A〉C位点多态性;采用χ^2检验比较各组间的差异。结果 KD组LTA基因rs2239704 A〉C位点的AC、AA、CC基因型分布和A、C等位基因频率与健康对照组比较差异无统计学意义（χ^2=3.3、0.81,均P〉0.05）,川崎病组合并CAL组与无CAL组LTA基因rs2239704 A〉C位点的基因型和等位基因频率比较差异亦无统计学意义（χ^2=0.71、0.04,均P〉0.05）。结论未发现LTA基因rs2239704 A〉C位点多态性与川崎病的发病及其CAL存在明显关联性。Objective To investigate the association of the locus rs2239704 A〉C polymorphisms oflymphotoxin-alpha （LTA） gene with Kawasaki disease （KD）. Methods The blood samples of 100 patientswith KD and 92 healthy controls were collected from 2000 to 2015, and the genomic DNA were extracted.The Sanger gene sequencing method was employed to detect the genotype of locus rs2239704 A〉Cpolymorphisms of LTA gene for these samples. The χ2 test was employed to detect the difference of thegroups. Results For locus rs2239704 A〉C polymorphism in LTA gene, there was no significantdifference between the KD patients and controls in genotyping frequencies of AC, AA, CC, respectively（χ2=3.3, P〉0.05）, and there was no significant difference in the allele frequencies of A and G between thetwo groups （χ2=0.81, P〉0.05）. There were no significant difference between the KD patients complicatedwith coronary artery injury group （CAL） and non coronary artery lesion group （NCAL） in genotype andallele frequency （χ2=0.71 and 0.04, P〉0.05）. Conclusion No association was found in genotype andallele frequency of the locus rs2239704 A〉C between the LTA gene with the risk of KD.

关 键 词：淋巴毒素Α 多态性 单核苷酸 黏膜皮肤淋巴结综合征 

分 类 号：R725.4[医药卫生—儿科]