一株高产丁二酮菌株的筛选与鉴定  

Screening and Identification of High Butanedione-Yielding Bacterial Strain

作　　者：粟桂蓉 周璐璐[1] 易浪波[1] 彭清静[1] 彭清忠[1] 

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《吉首大学学报（自然科学版）》2016年第2期38-42,共5页Journal of Jishou University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(31260003);吉首大学研究生科研创新项目(14JDY036)

摘　　要：以湖南省湘西地区土家腊肉中分离的41株细菌为出发菌,采用平板显色法和邻苯二胺比色法筛选高产丁二酮的微生物菌种.通过菌落形态观察、生理生化特征测定和基于16SrRNA基因序列分析,对分离菌株进行鉴定.结果表明,7株细菌能产生较高含量的丁二酮,其中菌株BCL-8产丁二酮量最高,在葡萄糖蛋白胨水培养基中发酵20h,丁二酮质量浓度达68.9mg/L,该菌经鉴定为地衣芽孢杆菌(Bacillus licheniformis).菌株BCL-8是一株优良的产丁二酮的微生物种质资源.To obtain the high butanedione-yielding bacterial strain,41 strains of bacteria isolated from traditional Tujia bacon were screened by way of V-P color reaction and 1,2-diaminobenzene colorimetric method. The isolated strains were identified by bacterial morphological, physiological and biochemical characteristics,and phylogenetic relationships based on 16S rRNA gene sequences. It was found that 7 strains of bacteria could produce higher content of butanedione,of which strain BCL-8 had the highest yield of butanedione. When strain BCL-8 had been fermented in glucose and peptone medium for 20 hours,butanedione content in fermentation liquor was determined to be 68.9 mg/L. Strain BCL-8 was identified as Bacillus licheniformis based on its morphological,physiological and biochemical characteris- tics,and 16S rRNA gene sequences analysis. The results showed that strain BCL-8 is a superior butanedione-producing microorganism.

关 键 词：丁二酮 地衣芽孢杆菌 筛选 鉴定 

分 类 号：Q939.111[生物学—微生物学]