利用斑马鱼模型研究芦荟大黄素对宫颈癌细胞SiHa的抑制作用  

Inhibitory effect of aloe emodine on cervical cancer cell line SiHa with zebrafish xenograft model

作　　者：王荣春[1] 韩利文[1] 陈锡强[1] 何秋霞[1] 王希敏[1] 彭维兵[1] 孙晨[1] 韩建[1] 刘可春[1] 

机构地区：[1]山东省科学院生物研究所山东省生物传感器重点实验室,山东济南250014

出　　处：《山东科学》2016年第2期14-18,共5页Shandong Science

基　　金：国家自然科学基金(31400979);山东省自主创新及成果转化专项(2014ZZCX02105);山东省重点研发项目(2015GSF121021)

摘　　要：通过细胞增殖实验(MTT)研究不同浓度芦荟大黄素(Aloe emodin,AE)对人宫颈癌细胞Si Ha生长的抑制作用,并将Si Ha细胞注入48 hpf的斑马鱼胚胎卵黄囊,建立斑马鱼移植瘤模型,然后用不同浓度AE进行处理,考察肿瘤细胞在斑马鱼体内的增殖和迁移情况。结果显示,AE对Si Ha细胞的生长抑制率随浓度和作用时间的增加而呈明显上升趋势,表现出一定的浓度-时间-效应依赖关系;体内实验表明AE对斑马鱼移植瘤的增殖和转移具有抑制作用。本研究表明,AE具有开发成抗宫颈癌药物的潜能,同时斑马鱼移植瘤模型也可以成为宫颈癌研究和药物评价的重要模型。We employ M-Fr to investigate inhibitory effect of different concentrations of aloe emodin （AE） on the proliferation of cervical cancer cell SiHa. We further establish zebrafish xenograft model by injecting SiHa into the yolk sac of 48 hpf zebrafish. The zebrafish is then treated with different concentrations of AE to investigate proliferation and migration of cancer cell in zebrafish. Results show that growth inhibition rate of AE on SiHa significantly increases with the increase of AE concentration and action time, a certain concentration-time-effect dependence. In vivo experiment shows that AE has inhibitory effect on the proliferation and metastasis of zebrafish xenograft. Our research indicates that AE has the potential of cervical anticancer drug. The zebrafish xenograft model can also be an important model of cervical cancer research and its drug assessment.

关 键 词：芦荟大黄素 斑马鱼 宫颈癌细胞 SIHA 移植瘤模型 

分 类 号：R965.1[医药卫生—药理学] R737.33[医药卫生—药学]