稻米垩白QTL定位分析  

QTL Analysis of Chalkiness in Rice

作　　者：彭强 张大双 吴健强 刘颖 黄培英 王际凤 朱速松 

机构地区：[1]贵州省水稻研究所,贵阳550006

出　　处：《种子》2016年第4期48-51,共4页Seed

基　　金：贵州省联合基金项目"水稻高密度遗传图谱构建和垩白QTL定位分析"(编号:黔科合LH字[2014]7689号);贵州省农科院专项"水稻优质;抗稻瘟病分子标记辅助选择育种研究"(编号:黔农科院院专项[2010]004号);贵州省科技计划项目"水稻中直链淀粉含量不育系MAS选育及利用"(编号:黔科合NY字[2010]3002号);贵州省科技计划项目"水稻种质改良创新及新品种选育与应用"(编号:黔科合重大专项字[2013]6023号);贵州茅台基金"水稻优质稻草和稻壳在茅台酒生产中的应用研究"(编号:黔科合茅科联字[2009]7012);国家高技术研究发展计划(863计划)项目"绿色超级稻新品种选育"(编号:2014AA 10A604)

摘　　要：以高垩白度品种V 20B和低垩白度品种CPSLO 17作为亲本,构建150个V 20B/CPSLO 17重组自交家系(RIL)为作图群体,进行稻米垩白QTL检测及其遗传效应分析。以垩白度为稻米垩白高低的表型数据,结合SLAF标签构建的分子连锁图谱,运用MapQTL 5软件检测到4个稻米垩白QTL,分别命名为qC-5 a、qC-5 b、qC-5 c和qC-5 d。这4个QTL的LOD值分别为4.02、4.09、3.94和4.1,对表型变异的解释率分别为11.6%、11.8%、11.2%和11.8%,且这4个QTL抗性等位基因都来自高垩白亲本V 20B。A mapping population of 150lines（recombination inbred lines,RIL）,derived from a cross between rice varieties V 20 Band CPSLO 17,was applied to analysis QTLs location of chalkiness trait and evaluated the genetic effects of two parents and 150 RILs.As the chalky degree of RIL populations were used as the phenotype data,the authors constructed a genetic linkage map based on a large number of SLAF markers,and detected 4QTLs（qC-5a、qC-5b、qC-5cand qC-5d）which controll chalkiness degree of rice on chromosome 5by using software Map QTL 5.Individual QTL（LOD=4.02,LOD=4.09,LOD=3.94,LOD=4.1）explained 11.6%、11.8%、11.2% and 11.8% of the observed phenotypic variance separately.All the four of detected QTLs alleles came from high-chalky parent V 20 B.

关 键 词：重组自交家系 垩白 作图群体 数量性状基因座 

分 类 号：S511[农业科学—作物学]