MAPK在不同生殖特性鲫鲤杂交鱼性腺组织中的表达分析  

Expression of MAPK in the Gonadal Tissue of Different Reproductive Characteristics of the Hybrid Crucian Carp

作　　者：王姿力[1] 骆瑜蓉 罗仲文[1] 王静[1] 肖亚梅[1] 

机构地区：[1]湖南师范大学生命科学学院,中国湖南长沙410081

出　　处：《生命科学研究》2016年第2期95-101,共7页Life Science Research

基　　金：国家自然科学基金资助项目(31172399);国家重点基础研究发展计划课题资助(2012CB722305)

摘　　要：运用Western-blot技术和免疫组织化学来检测丝裂原活化蛋白激酶(mitogen-activated protein kinases,MAPKs)家族成员细胞外调节蛋白激酶(extracellular regulated protein kinase,ERK)和c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)在不同生殖特性鲫鲤杂交鱼性腺组织中的表达。研究表明,JNK、ERK在鱼类性腺组织中均有较高量的表达,但在鱼类卵巢和精巢间、雌核发育二倍体鲫鲤和不同倍性鱼的性腺组织间,JNK或ERK的表达量并不存在明显的差异,而P-JNK在雌核发育二倍体鲫鲤性腺中的表达量远高于三倍体和四倍体鲫鲤的卵巢组织。此外,JNK和ERK在雌核发育二倍体鲫鲤早期性腺性原细胞中均有阳性表达。其中,JNK在10月龄雌核发育二倍体鲫鲤性腺中的阳性反应强度高于6、8月龄;而ERK在8月龄和10月龄的性腺中的阳性反应则弱于6月龄。结果表明JNK通路可能对雌核发育二倍体鲫鲤产生不减数配子具有重要调控作用。Western-blot and immunohistochemical staining were used to detect the expression of c-Jun N- terminal kinase （JNK） and extracellular regulated protein kinase （ERK） in gonad tissues of different ploidy crucian carps. It was found that JNK and ERK had high-level expression in fish gonad tissues and that no significant difference existed in the expression level of JNK or ERK between the ovary and testis as well as between diploid gynogenetic progeny and different ploidy level fishes. Expression of P-JNK in the gonad tis- sues of diploid gynogenetic progeny was much higher than that in triploid or tetraploid ovary. Expression of both JNK and ERK was detected positive in the spermatogonium of early gonad tissues in diploid gynogenet- ic progeny of different ages. JNK showed more strongly positive reaction in lO-month-old fish than in 6- and 8-month-old fish, while the positive reactions of ERK in 8-and 10-month-old fish were weaker than that in 6-month-old fish. The results indicated that JNK pathway might play an important role in regulation of unreduced gamete formation in diploid gynogenetic progeny.

关 键 词：雌核发育二倍体鲫鲤 不减数配子 JNK ERK 

分 类 号：Q28[生物学—细胞生物学]