DNA双链断裂修复过程中的重要蛋白53BP  

53BP1: a Key Protei n in Repair of Double-Strand Breaks

作　　者：张敏[1] 范久波[1] 邵金辉[1] 胡祁生[1] 于弘钧 

机构地区：[1]湖北文理学院医学院,中国湖北襄阳441053 [2]纽约州立大学石溪分校医学院,美国纽约11974

出　　处：《生命科学研究》2016年第2期162-165,共4页Life Science Research

基　　金：湖北省自然科学基金项目(2015CFC801);湖北省卫生和计划生育委员会一般项目(WJ2015MB189);湖北文理学院科研启动金项目(20131208)

摘　　要：DNA双链断裂(double-strand breaks,DSBs)修复对于保证基因组完整性以及维持细胞的平衡稳定性起着关键作用。p53结合蛋白1(p53-binding protein 1,53BP1)是针对产生的双链断裂损伤做出反应的重要调控因子。目前,研究人员对于53BP1被招募到受损的染色质上的过程,以及53BP1在DSBs修复过程中阻止同源重组(homologous recombination,HR)的同时推动非同源末端连接(non-homologous end-joining,NHEJ)的过程,已经有了新的认识。并且,近期的研究结果启发科学家们提出了一种新的模型,即53BP1的招募需要直接识别DSBs特异性的组蛋白密码,而53BP1发挥作用时的通路选择则与BRCA1蛋白的拮抗作用有关。结合近年来有关53BP1的研究进展,主要综述了53BP1的结构与功能特点,其作为调控因子在DSBs修复过程中发挥的作用,以及53BP1达到有效聚集的方式。The repair of DNA double-strand breaks （DSBs） is crucial to preserve genomic integrity and maintain cellular homeostasis, p53-binding protein 1 （53BP1） is an important regulator in response to DSBs. So far, new insights have been gained into the mechanism underlying the recruitment of 53BP1 to damaged chromatin. Also, new evidences have been revealed how 53BP1 promotes non-homologous end-joining-mediated DSBs repair while preventing homologous recombination. In addition, based on recent studies, the new model has been put forward that 53BP1 recruitment requires the direct recognition of a DSBs-specific his- tone code and its pathway choice is mediated by mutual antagonism with BRCA1. Here, the structural and functional characteristics of 53BP1 are reviewed and an overview of its role as a mediator in DSBs repair pathway is presented, as well as its recruitment to damaged chromatin.

关 键 词：DNA双链断裂 p53结合蛋白1 通路选择 标志识别 

分 类 号：Q71[生物学—分子生物学]