巴戟天及其不同炮制品对脾肾阳虚模型大鼠的改善作用比较  

Comparison of Improvement Effect of Morinda officinalis and Its Processed Products on Spleen-kidney Yang Deficiency Model Rats

作　　者：史辑[1,2,3] 景海漪[1] 黄玉秋[1] 范亚楠[1] 贾天柱[1,2,3] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]国家中医药管理局炮制原理解析重点研究室,辽宁大连116600 [3]辽宁省中药炮制工程技术研究中心,辽宁大连116600

出　　处：《中国药房》2016年第13期1756-1758,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81473350)

摘　　要：目的:比较巴戟天及其不同炮制品对脾肾阳虚模型大鼠的改善作用。方法:将80只大鼠随机分为正常组(生理盐水)、模型组(生理盐水)、辅料组(甘草汁,0.063 g/kg)、全鹿丸组(阳性药物,1.89 g/kg)和巴戟天、巴戟肉、盐巴戟、制巴戟组[给药剂量均为1.05 g(生药)/kg]。除正常组外,其余各组大鼠均采用ig大黄水煎液+im氢化可的松复制脾肾阳虚模型,并同时ig相应药物,每天1次,连续15 d。测定血清中睾酮(T)、雌二醇(E_2)、环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、三碘甲状腺原氨酸(T_3)、甲状腺素(T_4)、促肾上腺素皮质激素(ACTH)水平,并计算E_2/T、cAMP/cGMP比值。结果:与正常组比较,模型组大鼠血清中T、T_3、T_4、cGMP水平降低,E_2、cAMP、ACTH水平及E_2/T、cAMP/cGMP比值升高(P<0.05或P<0.01)。与模型组比较,各给药组大鼠血清中T水平升高,E_2、cAMP、ACTH水平及cAMP/cGMP比值降低(P<0.05或P<0.01),且以盐巴戟组大鼠血清中T水平最高、E_2/T比值最低,制巴戟组大鼠血清中E_2、cAMP、ACTH水平及cAMP/cGMP比值最低;巴戟天组和制巴戟组大鼠血清中cGMP水平升高(P<0.01);巴戟天组、盐巴戟组和制巴戟组大鼠血清中T_3水平及各给药组大鼠血清中T4水平升高(P<0.05或P<0.01),且以制巴戟组升高最为显著。结论:在巴戟天及其不同炮制品中,制巴戟对脾肾阳虚大鼠的改善作用更明显。OBJECTIVE：To compare the improvement effects of Morinda officinalis and its processed products on rats with spleen-kidney Yang deficiency. METHODS：80 rats were randomly divided into normal group（normal saline）,model group（normal saline）,excipients group（succus liquiritiae,0.063 g/kg）,Quanlu pill group（positive drug,1.89 g/kg）,M. officinalis group,M. officinalis without wood,M. officinalis processed by salt and M. officinalis processed by licorice-juice groups [1.05 g（crude drug）/kg]. Those groups were given Rheum palmatum water solution intragastrically and hydrocortisone intramuscularly to induce spleen-kidney Yang deficiency model,and given relevant medicine intragastrically once a day for consecutive 15 d. The levels of testosterone（T）,estradiol（E_2）,c AMP,c GMP,triiodothyronine（T_3）,thyroxine（T_4）,adrenal cortex hormone（ACTH）in the serum were measured,and E_2/T and c AMP/c GMP were calculated. RESULTS：Compared with normal group,the serum level of T,T_3,T_4 and c GMP decreased in model group,while the levels of E_2,c AMP and ACTH,E_2/T and c AMP/c GMP increased（P〈0.05 or P〈0.01）. Compared with model group,serum level of T increased in treatment groups,while serum levels of E_2,c AMP,ACTH and c AMP/c GMP decreased（P〈0.05 or P〈0.01）;serum level of T was highest and E_2/T was the lowest in M. officinalis processed by salt group;serum level of E_2,c AMP,ACTH and c AMP/c GMP were the lowest in M. officinalis processed by licorice-juice group;serum level of c GMP increased in M. officinalis group and M. officinalis processed by licorice-juice group（P〈0.01）;serum levels of T_3 in M. officinalis,M. officinalis processed by salt and M. officinalis processed by licorice-juice groups,and T_4 increased in all treatment groups（P〈0.05 or P〈0.01）,especially in M. officinalis processed by licorice-juice group. CONCLUSIONS：M. officinalis processed by licorice-juice is better in improving spleen-kidney Yang deficiency,compared to M. officinalis and it

关 键 词：巴戟天 炮制品 脾肾阳虚模型 睾酮 雌二醇 三碘甲状腺原氨酸 甲状腺素 促肾上腺素皮质激素 大鼠 

分 类 号：R285[医药卫生—中药学]