对映-贝壳杉烷型二萜Leukamenin E对人早幼粒白血病HL-60细胞分化的诱导作用  

Induction Effects of Ent-kaurane Diterpene Leukamenin E on the Differentiation of Human Promyelocytic Leukemia HL-60 Cells

作　　者：丁兰[1] 李佩蔚 孔花青[1] 陈宗儒 刘国安[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《中国药房》2016年第13期1771-1774,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.30960464)

摘　　要：目的：研究对映-贝壳杉烷型二萜Leukamenin E对人早幼粒白血病HL-60细胞分化的诱导作用。方法：采用台盼蓝染色法、吉姆萨染色法、NBT还原力测定法、荧光颗粒吞噬法及流式细胞术分别检测0（空白对照）、0.3、0.6、0.9、1.2μmol/L的Leukamenin E及1.2μmol/L全反式维甲酸（ATRA）作用于HL-60细胞24、48、72、96 h后对细胞数目的影响,以及作用72 h后细胞核形态、NBT还原能力（以NBT阳性细胞率计）、细胞吞噬能力（以荧光探针P的荧光强度计）以及细胞表面抗原CD11b表达的改变情况。结果：与空白对照比较,0.3-1.2μmol/L的Leukamenin E及1.2μmol/L的ATRA作用48、72、96 h后HL-60细胞数目减少,且作用72 h后带状核细胞和分叶状核细胞数目增加（P〈0.01）,细胞NBT阳性细胞率和细胞表面CD11b表达水平增加（P〈0.01）,P荧光强度增强。结论：Leukamenin E可诱导HL-60向成熟粒细胞分化,与白血病分化治疗剂ATRA具有相似的分化诱导特点。OBJECTIVE：To study the induction effect of ent-kaurane diterpenoid Leukamenin E on the differentiation of human promyelocytic leukemia HL-60 cells. METHODS：Trypan blue staining,Giemsa＇s staining,NBT reduction test,fluorescent particles phagocytosis test and flow cytometry were used to detect the number of HL-60 cells after treated with 0,0.3,0.6,0.9 and 1.2 μmol/L Leukamenin E and 1.2 μmol/L all-trans retinoic acid（ATRA）for 24,48,72 and 96 h,and the cellular nucleus morphology,NBT-reducing ability（by NBT positive cell rate）,phagocytic ability（by fluorescence intensity of fluorescence probe P）and the expression of antigen CD11 b after treated for 72 h were observed. RESULTS：Compared with blank control,the number of HL-60 cells decreased after treated with 0.3-1.2 μmol/L Leukamenin E and 1.2 μmol/L ATRA for 48,72 and 96 h;the number of band nucleus and segmented nuclei increased after treated for 72 h（P〈0.01）;the rate of positive NBT cells and the expression of CD11 b increased（P〈0.01）; the fluorescence intensity of P strengthened. CONCLUSIONS：Leukamenin E could induce the differentiation of HL-60 cells to mature granulocyte,which is similar to the effects on HL-60 cells induced by leukemia differentiation therapeutic agent ATRA.

关 键 词：对映-贝壳杉烷型二萜 Leukamenin E 人早幼白血病HL-60细胞 分化 

分 类 号：R965[医药卫生—药理学]