壮骨伸筋胶囊对泼尼松致骨质疏松模型大鼠的改善作用及其机制研究  

Study on Improvement Effects of Zhuanggu Shenjin Capsules on Prednisone-induced Osteoporosis Model Rats and Its Mechanism

作　　者：李丽娟 孙晓丽 苏海峰 段重高 周亚伟 

机构地区：[1]北大世佳科技开发有限公司,北京100084

出　　处：《中国药房》2016年第13期1788-1790,共3页China Pharmacy

基　　金：吉林省科技发展计划项目(No.20130302025YY)

摘　　要：目的:研究壮骨伸筋胶囊对骨质疏松模型大鼠的改善作用及其机制。方法:将72只SD大鼠随机分为6组,即空白对照组(生理盐水)、模型组(生理盐水)、骨疏康颗粒组[阳性药物,3 g(生药)/kg]和壮骨伸筋胶囊低、中、高剂量组[1.4、2.7、5.4 g(生药)/kg]。除空白对照组ig生理盐水外,其余各组大鼠于每周一、四ig醋酸泼尼松片(4.5 mg/kg)复制骨质疏松模型,并同时于每周一至六ig相应药物,连续13周。测定大鼠股骨骨密度、骨形态计量指标[骨小梁体积百分比、骨小梁矿化率(MAR)和骨皮质矿化率(mAR)]、肾组织中肌节同源盒基因Msx-2 mRNA及其蛋白的表达。结果:与空白对照组比较,模型组大鼠骨密度、骨小梁体积百分比降低,mAR升高,肾组织中Msx-2 mRNA及其蛋白表达减弱(P<0.05或P<0.01)。与模型组比较,骨疏康颗粒组和壮骨伸筋胶囊高剂量组大鼠骨小梁体积百分比升高、m AR降低,肾组织中Msx-2 mRNA表达增强;各给药组大鼠骨密度增加、Msx-2蛋白表达增强;中剂量组骨小梁体积百分比升高,以上差异均有统计学意义(P<0.05或P<0.01);MAR差异无统计学意义(P>0.05)。结论:壮骨伸筋胶囊对泼尼松致大鼠骨质疏松有一定的改善作用,其机制可能与上调肾组织中Msx-2基因的表达有关。OBJECTIVE：To study the improvement effects of Zhuanggu shenjin capsules（ZGSJC）on prednisone-induced osteoporosis model rats and its mechanism. METHODS：72 SD rats were randomly divided into 6 groups as blank control group（normal saline）,model group（normal saline）,Gushukang granule group [positive drug,3 g（crude drug）/kg] and ZGSJC low-dose,medium-dose and high-dose groups [1.4,2.7,5.4 g（crude drug）/kg]. Except for intragastric administration of normal saline in blank control group,other groups were given Prednisone acetate tablet 4.5 mg/kg intragastricallly every Monday and Thursday to induce osteoporosis model,and given relevant medicine intragastrically from Monday to Saturday for consecutive 13 weeks. Bone density（BMD）,bone histomorphometry indicators [volume percentage of bone trabecula,mineralization rate of bone trabecular（MAR）and mineralization rate of bone cortical（mAR）] and the expression of muscle segment homeebox gene Msx-2 mRNA and protein were all determined. RESULTS：Compared with blank control group,BMD,volume percentage of bone trabecula and the expression of Msx-2 mRNA and protein in renal tissue decreased,while m AR increased in model group（P〈0.05 or P〈0.01）.Compared with model group,volume percentage of bone trabecula and the expression of Msx-2 mRNA in renal tissue increased in Gushukang granule group and ZGSJC high-dose group,while mAR decreased;BMD and the expression of Msx-2 protein increased in treatment groups,as well as the volume percentage of bone trabecula increased in ZGSJC medium-dose group,with statistical significance（P〈0.05 or P〈0.01）,while the difference of MAR without statistic significance（P〉0.05）. CONCLUSIONS：ZGSJC can protect rats against prednisone-induced osteoporosis,and its mechanism may be associated with the up-regulation of Msx-2 expression in renal tissue.

关 键 词：壮骨伸筋胶囊 骨质疏松 醋酸泼尼松 骨组织形态计量 肌节同源盒基因Msx-2 大鼠 

分 类 号：R285[医药卫生—中药学]