虎石灌肠颗粒的体外抑菌作用考察  

Study on Antibacterial Effect of Hushi Enema Granules in vitro

作　　者：吴嵩[1] 廖佑荣 谭璐[1] 张广求[1] 

机构地区：[1]黄冈市中心医院药剂科,湖北黄冈438000

出　　处：《中国药房》2016年第13期1807-1810,共4页China Pharmacy

摘　　要：目的：考察虎石灌肠颗粒的体外抑菌作用。方法：采用试管二倍稀释法测定虎石灌肠颗粒药液[初始质量浓度为1 200 mg（生药）/ml]对金黄色葡萄球菌、大肠埃希菌、沙门菌、志贺氏痢疾杆菌、福氏痢疾杆菌的最低抑菌浓度（MIC）;采用琼脂培养基平板法测定其最低杀菌浓度（MBC）;选择4 MIC和2 MIC药液测定杀菌速度（菌液浓度均为1.5×10^（-5） CFU/ml）,并绘制杀菌曲线（KC_S）图。结果：虎石灌肠颗粒药液对金黄色葡萄球菌、大肠埃希菌、沙门菌、志贺氏痢疾杆菌、福氏痢疾杆菌的MIC分别为18.75、150、150、75、150 mg（生药）/ml,MBC分别为37.50、150、150、75、150 mg（生药）/ml;KC_S图显示,4MIC、2MIC药液对5种受试菌株均有较好的杀灭作用,全部杀灭所需时间分别为24、1、1、7、1 h和24、7、3、7、7 h。结论：虎石灌肠颗粒具有较强的体外抑菌作用。OBJECTIVE：To study antibacterial effect of Hushi enema granules in vitro. METHODS：Minimal inhibitory concentration（MIC）of Hushi enema granules [initial concentration of 1 200 mg（crude drug）/ml] to Staphylococcus aureus,Escherichia coli,Salmonella,Shigella dysenteriae and Shigella flexneri were detected by tube double dilution method. The minimal bactericidal concentration（MBC） were determined by agar medium plate method;4 MIC and 2 MIC Hushi enema granules solution were selected to determine sterilizing speed（bacterium concentration of 1.5 × 10-5CFU/ml）and draw time-kill curves（KC_S）. RESULTS：MIC of Hushi enema granules to S. aureus,E. coli,Salmonella,S. dysenteriae and S. flexneri were 18.75,150,150,75 and 150 mg（crude drug）/ml,respectively;MBC of Hushi enema granules were 37.50,150,150,75 and 150 mg（crude drug）/ml,respectively. Both 4 MIC and 2 MIC solution had good sterilizing effects on 5 bacterial strains. KCs of 4 MIC and 2 MIC solution sterilizing 5 bacterial strains were 24,1,1,7,1 h and 24,7,3,7,7 h,respectively. CONCLUSIONS：Hushi enema granules show a strong antibacterial effect in vitro.

关 键 词：虎石灌肠颗粒 体外抑菌作用 最低抑菌浓度 最低杀菌浓度 杀菌曲线 

分 类 号：R285.5[医药卫生—中药学] R965[医药卫生—中医学]