小鼠抗缺氧活性实验结合正交试验优选藏紫菀中总黄酮的提取工艺  

Optimization of the Extraction Technology of Total Flavonoids from Aster souliei by Orthogonal Test Combined with Anti-hypoxia Activity Test of Mice

作　　者：何蕾[1] 马慧萍[1] 李兰茹[1] 贾正平[1] 

机构地区：[1]兰州军区兰州总医院药材科,兰州730050

出　　处：《中国药房》2016年第13期1832-1835,共4页China Pharmacy

基　　金：全军医药卫生科研基金课题(No.CLZ11JA06);甘肃省自然科学研究基金计划项目((No.1107RJZA100)

摘　　要：目的:优选藏紫菀中总黄酮的提取工艺。方法:通过小鼠常压密闭缺氧耐力实验筛选提取藏紫菀中总黄酮的溶剂(水、60%乙醇、95%乙醇);以浸膏得率和总黄酮含量为指标,采用单因素和正交试验筛选提取溶剂加入量、提取时间和提取次数的最优水平,并进行验证试验。结果:提取溶剂为水时小鼠存活时间最长;最优提取工艺为加12倍量水,回流提取2次,每次2 h;验证试验中总黄酮平均含量为29.70%(RSD=0.54%,n=3)、浸膏得率平均为24.58%(RSD=0.48%,n=3)。结论:优选的提取工艺条件稳定、可行,可为藏紫菀总黄酮的开发利用提供科学依据。OBJECTIVE：To optimize the extraction technology of total flavonoids from Aster souliei. METHODS：Atmospheric pressure closed hypoxia tolerance test of mice was used to screen the solvents（water,60% ethanol,95% ethanol）for the extraction of total flavonoids from A. souliei. Using extract yield and the content of total flavones as index,single factor test and orthogonal test were used to screen the amount of solvent,extraction time and extraction times. The validation test was also conducted. RESULTS：When the extraction solvent was water,the survival time of the mice was the longest. The optimized extraction technology was as follows as 12-fold water,extracting for 2 times,lasting for 2 h each time;average content of total flavonoids in validation test was 29.70%（RSD=0.54%,n=3）and extract yield was 24.58%（RSD=0.48%,n=3）. CONCLUSIONS：The optimized extraction technology is stable and feasible,and can provide scientific evidence for the development and utilization of total flavonoids from A. souliei.

关 键 词：藏紫菀 总黄酮 小鼠 常压密闭缺氧耐力实验 含量测定 正交试验 提取工艺 

分 类 号：R283[医药卫生—中药学]