氯虫苯甲酰胺抗性小菜蛾的产卵行为和交配节律观察  

Timing of oviposition and mating in chlorantraniliprole-resistant Plutella xylostella(L.)

作　　者：王海慧[1,2,3] 章金明[2,3] 涂芹 刘桂英[2,3] 吕要斌[1,2,3] 

机构地区：[1]杭州师范大学生命与环境科学学院,杭州310036 [2]浙江省农业科学院植物保护与微生物研究所 [3]浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地,杭州310021

出　　处：《应用昆虫学报》2016年第2期264-272,共9页Chinese Journal of Applied Entomology

基　　金：公益性行业专项(201103021);“十二五”国家科技支撑计划项目(2012BAD19B06);浙江省农科院创新提升工程课题(2014CX026)

摘　　要：【目的】为了明确氯虫苯甲酰胺抗性小菜蛾Plutella xylostella(L.)的交配节律、产卵节律和产卵部位偏好是否发生变化,以及这些行为变化是否有利于小菜蛾抗药性的形成。【方法】采用室内观察的方法,比较了小菜蛾氯虫苯甲酰胺抗性品系与室内品系在产卵行为和交配节律上的差异。【结果】(1)抗性品系在甘蓝植株上产卵时偏好选择中下部叶片,L1-L5叶上卵量占总卵量的比例,抗性品系显著高于室内品系;抗性品系在叶背面产卵的比例也高于室内品系,但差异不显著;而在心叶、叶柄和茎干部位3个品系间的产卵比例基本相当;(2)3个品系交配比例最高的时间段均在0:00—6:00,但交配比率第二高的时间段3个品系间存在差异,室内品系(29.40%)和中抗品系(25.91%)出现在18:00—24:00,而高抗品系(28.67%)出现在12:00—18:00;(3)室内品系、中抗品系和高抗品系白天产卵量占总产卵量的比例分别为39.49%、37.57%和42.67%,高抗品系白天产卵比例明显增加。但3个品系在4个时间段产卵从多到少规律一致,依次是0:00—6:00,12:00—18:00,18:00—24:00,6:00—12:00。【结论】抗性品系在甘蓝植株上产卵时,选择的部位更靠近植株的中下部,趋向于叶片背部。抗性品系和室内品系的交配节律也存在一定的差异,交配高峰期有一定程度的错开。氯虫苯甲酰胺抗性品系的这些行为变化有利于抗性基因的纵向传递,但是否影响抗性形成的速度需要进一步研究。[Objectives] To clarify whether differences in the timing of mating and oviposition, or oviposition preferences, exist between a chlorantraniliprole-resistant, and a laboratory, strain of Plutella xylostella, and whether differences in these behaviors are conducive to the evolution of insecticide resistance. [Methods] The timing of mating and oviposition in a laboratory（LAB） and two chlorantraniliprole-resistant strains（a medium resistant（MR） strain and a high resistant（HR） strain）, were compared under laboratory conditions. [Results]（1） The resistant strains preferred to lay eggs on the middle underside of the leaves of cabbages and laid more eggs than the LAB strain on leaves L1-L5. Resistant strains laid a greater proportion of eggs on lower, than on higher, leaves than the LAB strain, but there was no significant difference in the proportions of eggs laid by the different strains on growing points（GP）, stem（S） and petioles（P）.（2） Most mating activity in all three strains took place between 00：00-06：00, but the timing of the second mating peak differed between strains; that of the LAB（29.40%） and MR strains（25.91%） occurring between 18：00-24：00, whereas that of the HR strain（28.67%） occurred from 12：00-18：00.（3） The percentages of eggs laid in the daytime by the LAB, MR and HR strains were 39.49%, 37.57% and 42.67%, respectively, but the proportion of eggs laid in the daytime by the HR-strain was significantly higher. All three strains were similar in the timing of oviposition; oviposition periods could be ranked in descending order of egg abundance from, 00：00-06：00, 12：00-18：00, 18：00-24：00 to 06：00-12：00. [Conclusion] Resistant strains prefer to lay their eggs on the mid-underside of lower cabbage leaves. There were some differences in the timing of mating and oviposition between the resistant and LAB strains. Furthermore, the peak mating periods of the HR and LAB strain were partly staggered. These behavioral differences facili

关 键 词：小菜蛾 氯虫苯甲酰胺 抗性品系 产卵部位 交配和产卵节律 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]