气相色谱法测定复方丹参滴丸给药制剂中异龙脑和龙脑含量  

Determination of Iso- borneol and borneol in compound danshen dripping pill by capillary gas chromatography

作　　者：刘彦莉[1] 王萍[1] 张磊[1] 孙巍[1] 米晓兰[1] 

机构地区：[1]天津天士力制药股份有限公司,天津300402

出　　处：《天津药学》2016年第2期13-15,共3页Tianjin Pharmacy

摘　　要：目的:使用气相法测定复方丹参滴丸给药制剂中异龙脑、龙脑含量。方法:采用气相色谱法测定,乙酸乙酯为溶剂,水杨酸甲酯为内标。色谱柱为HP-5型5%PHMEsiloxana石英毛细管柱(30 m×0.32 mm,0.25μm),载气为氮气,分流比10∶1。FID检测器,检测器温度:250℃;进样口温度:200℃;柱温:100℃保持12 min。进样体积1μl。结果:异龙脑在0.010~0.30 mg/ml(r=0.999 9)、龙脑在0.020~0.60 mg/ml范围内呈良好线性关系,异龙脑平均回收率为98.51%,RSD为0.67%;龙脑平均回收率为98.54%,RSD为0.95%。结论:方法快速、准确、可靠,可用于复方丹参滴丸给药制剂中异龙脑、龙脑含量的检测。Objectives： To establish a method of a capillary gas chromatography for determination of iso - borneo1 and borneol contents in compound Danshen dripping pill. Methods： a gas chormatographic method was developed with HP -5 capillary column { 30 m ×0.32 mm, 0.25 μm ） as stationary phase and nitrogen as carrier gas. The compounds were determined with FID. The temperature of the injector and detector were set at 200~C and 250 ~C, respectively. Column temperature was at 100 ~C for 12 mi- nutes. The injection volume was 1 i^1. Results： The calibration curves showed a good linear relationship within the range of 0.010 ~ 0.30 mg/ml of iso -borneol and 0.020 ~ 0.60 mg/ml of borneol with good precision and accuracy, respectively. 98.51% of isoborneol and 98.54% of borneol was recovered from the samples, respectively. Conclusions ： With excellent aceuaracy, high sensitivity and reliability, the method can be applied to determine the contents of iso - and borneol in compound Danshen dripping hill.

关 键 词：石英毛细管气相色谱 复方丹参滴丸给药制剂 异龙脑 龙脑 

分 类 号：R927.2[医药卫生—药学]