心可舒与氟伐他汀抑制兔动脉粥样硬化黏附分子的表达及炎性反应  

Xinkeshu and fluvastatin inhibit expression of adhesive molecules and inflammation in plaque of atherosclerosis in rabbit

作　　者：黑乃豪 徐飞飞[2] 曹新冉 徐慧[2] 陈旭[2] 郝青青[2] 牟林茂[1,2] 董波[1,2] 

机构地区：[1]山东中医药大学,山东济南250355 [2]山东省立医院心内科,山东济南250012

出　　处：《基础医学与临床》2016年第5期590-593,共4页Basic and Clinical Medicine

基　　金：山东省自然科学基金(03BS037)的部分赞助(DFCMDA201213)

摘　　要：目的观察心可舒及氟伐他汀对兔动脉粥样硬化(AS)斑块血管细胞黏附分子(VCAM-1)的影响及其机制。方法将雄性新西兰大白兔随机分为对照组、高脂AS模型组、氟伐他汀组及心可舒组,每组9只。通过HE染色评估粥样斑块的程度。应用免疫组化检测巨噬细胞、基质金属蛋白酶9(MMP-9)和α-平滑肌肌动蛋白(α-SMA)的表达,蛋白免疫印迹法分析斑块VCAM-1的表达水平,并检测氧自由基SOD及MDA的含量。结果心可舒及氟伐他汀组VCAM-1的表达明显低于高脂组的水平(P<0.01),巨噬细胞、MMP-9及α-SMA的水平亦明显低于高脂组(P<0.01),SOD的含量明显升高(P<0.01)。结论心可舒及氟伐他汀通过抗氧自由基及抗炎作用保护兔血管内皮及抗动脉粥样硬化。Objective To observe the effect of Xinkeshu （XKS） and fluvastatin on adhesive molecules and inflammation in plaque of atherosclerosis in rabbits. Methods New Zealand white rabbits were randomly divided into four groups ： control group, high-fat group, fluvastatin group and Xinkeshu （XKS） group （9/per group）. The atherosclerode plaques were evaluated by HE staining. Expression of MMP-9, macrophage and α-SMA in atherosclerotic plaques were detected by immunohistochemistry. The SOD and MDA were also measured. Results Compared with high-fat group,the expressions of VCAM-1 in atheroselerotic plaques was significantly decreased with the treatment of XKS and fluvastatin（P 〈 0.01 ）. The expression of MMP-9 ,macrophage infiltration andα-SMA was also lower in the XKS group and fluvastatin group than that in high-fat group （P 〈 0. 01 ）, while serum SOD activity increased （P 〈 0.01）. Conclusions XKS and fluvastatin play important roles in the protection of endothelial function and anti-atherosclerosis effect by its anti-inflammatory and anti-oxygen radical.

关 键 词：动脉粥样硬化 心可舒 氟伐他汀 黏附分子 

分 类 号：R543.5[医药卫生—心血管疾病]