腹水COX-2 mRNA的表达及意义  

Expression and Significance of COX-2 mRNA in Ascites

作　　者：廖素环[1] 李啸峰[1] 江堤[2] 路静[1] 邓惠钊 何映[1] 龙小华[1] 

机构地区：[1]中山大学附属第五医院消化内科,广东珠海519000 [2]东莞东华医院消化内科,广东东莞523000 [3]东莞东华医院肾内科,广东东莞523000

出　　处：《中山大学学报（医学科学版）》2016年第2期254-259,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(2014A020212387);珠海市科技计划项目(2014D0401990005)

摘　　要：【目的】通过检测腹水COX-2 m RNA的表达水平,探讨COX-2 m RNA对腹水鉴别诊断的价值和意义。【方法】收集2014年6月至2015年2月期间到我院就诊并明确诊断的恶性腹水34例,良性腹水15例。采用实时荧光定量PCR方法检测腹水中COX-2 m RNA的表达水平,比较COX-2 m RNA在良恶性腹水中表达的差异,及在不同原发疾病中的表达情况。【结果】34例恶性腹水及15例良性腹水均扩增出COX-2,良恶性腹水中COX-2 m RNA的相对表达水平与性别、年龄构成比无关系,差异无统计学意义(P>0.05)。恶性腹水和良性腹水COX-2 m RNA的表达水平分别为7.93(5.04,16.58)和0.95(0.57,1.79),即恶性腹水COX-2 m RNA的表达水平显著高于良性腹水,差异有统计学意义(P<0.05)。恶性腹水COX-2m RNA的表达水平在不同原发疾病中差异无统计学意义(P>0.05),良性腹水COX-2 m RNA表达水平在肝硬化和结核性腹膜炎中分别为1.55(0.55,2.55)和0.87(0.61,1.38),差异无统计学意义(P>0.05)。【结论】COX-2 m RNA在恶性腹水中的表达水平显著高于良性腹水,在鉴别诊断腹水性质中具有重要的参考价值。[ Objective ] To explore the value and significance of COX-2 mRNA in differential diagnosis of ascites by detecting the expression level of COX-2 mRNA in ascites. [Method] 49 cases of ascites were collected from June 2014 to Febniary 2015 in oar hospital, including 43 cases of malignant ascites and 15 cases of benign ascites. Expression level of COX-2 mRNA in ascites were detected by fluorescence quantitative PCR technology, compared the difference of expression level of COX-2 mRNA in malignant ascites and benign ascites, and the expression level in different kind of cause. [ Results ] 34 cases of malignant ascites and 15 cases of benign ascites were all amplified COX-2 and GAPDH. The expression level of COX-2 mRNA in malignant and benign ascites had no relationship with gender and age （P 〉 0.05）. Expression level of COX-2 mRNA in malignant ascites and benign ascites were 7.93 （5.04, 16.58） and 0.95 （0.57, 1.79） respectively, difference being statistically significant （P 〈 0.05）. Expression level of COX-2 mRNA in malignant ascites were different in different kinds of primary tumors （P 〉 0.05 ）. Expression level of COX-2 mRNA in benign ascites were not significant difference between liver cirrhosis and tuberculous peritonitis （P 〉 0.05）. [Conclusion] High COX-2 mRNA expression was found in malignant ascites, which were higher than in benign ascites, it will be great significance in the differential diagnosis of malignant ascites and benign ascites.

关 键 词：腹水 COX-2 MRNA 荧光定量PCR 肿瘤 

分 类 号：R57[医药卫生—消化系统]