Transgenic plants expressing the AalT/GNA fusion protein show increased resistance and toxicity to both chewing and sucking pests  

作　　者：Shu-Min Liu Jie Li Jin-Qi Zhu Xiao-Wei Wang Cheng-Shu Wang Shu-Sheng Liu Xue-Xin Chen Sheng Li 

机构地区：[1]Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai [2]State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou, China

出　　处：《Insect Science》2016年第2期265-276,共12页昆虫科学（英文版）

基　　金：This study was supported by the National Program for the Development of New Transgenic Species of China （2014ZX08010-016B to S Li and 2011ZX08011-006 to XXC） and the National Science Foundation of China （31302034 to Shumin Liu）. We thank Nature Publishing Group for polishing the English language.

摘　　要：The adoption of pest-resistant transgenic plants to reduce yield losses and de- crease pesticide use has been successful. To achieve the goal of controlling both chewing and sucking pests in a given transgenic plant, we generated transgenic tobacco, Arabidopsis, and rice plants expressing the fusion protein, AalT/GNA, in which an insecticidal scor- pion venom neurotoxin （Androctonus australis toxin, AalT） is fused to snowdrop lectin （Galanthus nivalis agglutinin, GNA）. Compared with transgenic tobacco and Arabidop- sis plants expressing AaIT or GNA, transgenic plants expressing AalT/GNA exhibited increased resistance and toxicity to one chewing pest, the cotton bollworm, Helicoverpa armigera. Transgenic tobacco and rice plants expressing AalT/GNA showed increased resistance and toxicity to two sucking pests, the whitefly, Bemisia tabaci, and the rice brown planthopper, Nilaparvata lugens, respectively. Moreover, in the field, transgenic rice plants expressing AalT/GNA exhibited a significant improvement in grain yield when infested with N. lugens. This study shows that expressing the AalT/GNA fusion protein in transgenic plants can be a useful approach for controlling pests, particularly sucking pests which are not susceptible to the toxin in Bt crops.The adoption of pest-resistant transgenic plants to reduce yield losses and de- crease pesticide use has been successful. To achieve the goal of controlling both chewing and sucking pests in a given transgenic plant, we generated transgenic tobacco, Arabidopsis, and rice plants expressing the fusion protein, AalT/GNA, in which an insecticidal scor- pion venom neurotoxin （Androctonus australis toxin, AalT） is fused to snowdrop lectin （Galanthus nivalis agglutinin, GNA）. Compared with transgenic tobacco and Arabidop- sis plants expressing AaIT or GNA, transgenic plants expressing AalT/GNA exhibited increased resistance and toxicity to one chewing pest, the cotton bollworm, Helicoverpa armigera. Transgenic tobacco and rice plants expressing AalT/GNA showed increased resistance and toxicity to two sucking pests, the whitefly, Bemisia tabaci, and the rice brown planthopper, Nilaparvata lugens, respectively. Moreover, in the field, transgenic rice plants expressing AalT/GNA exhibited a significant improvement in grain yield when infested with N. lugens. This study shows that expressing the AalT/GNA fusion protein in transgenic plants can be a useful approach for controlling pests, particularly sucking pests which are not susceptible to the toxin in Bt crops.

关 键 词：AalT AalT/GNA chewing pest GNA fusion protein pest control RESISTANCE sucking pest toxicity transgenic plant 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S763[农业科学—植物保护]