去卵巢大鼠骨组织中肿瘤坏死因子α、白细胞介素1β及白细胞介素6水平与骨质疏松的关系  

Relationship between the levels of tumor necrosis factor alpha, interleukin-1beta and interleukin 6 of bone tissue and osteoporosis in ovariectomized rats

作　　者：朱书涛[1] 刘洋[1] 张明辉[1] 王晓[1] 

机构地区：[1]河南大学淮河医院骨科,河南省开封市475000

出　　处：《中国组织工程研究》2016年第15期2206-2211,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究发现,肿瘤坏死因子α、白细胞介素1β、白细胞介素6分泌量与骨质疏松的形成之间联系紧密。目的:进一步分析去卵巢大鼠骨组织中肿瘤坏死因子α、白细胞介素1β、白介细胞素6水平及其与骨质疏松形成的关系。方法:3个月龄雌性SD大鼠60只,根据随机数表法,将大鼠分为对照组和研究组,每组30只。研究组大鼠通过手术去除卵巢,并建立骨质疏松模型,而对照组大鼠保留卵巢,腹腔注射等量生理盐水,实验周期12周。造模后1周检测大鼠血清中雌二醇水平,采用骨密度仪测量大鼠骨密度,并检测骨组织中肿瘤坏死因子α、白介细胞素1β及白介细胞素6水平,分析细胞因子和骨密度之间的相关性。结果与结论:研究组血清中雌二醇水平明显低于对照组(P=0.007);研究组大鼠腰椎和股骨骨密度显著低于对照组(P=0.006,0.004);研究组骨小梁和成骨细胞占视野面积比例明显低于对照组,而破骨细胞占视野面积比例显著高于对照组(P=0.037,0.029,0.044);与对照组相比,研究组骨组织中肿瘤坏死因子α、白介细胞素1β及白介细胞素6水平明显升高(P=0.032,0.031,0.025);研究组大鼠骨组织中肿瘤坏死因子α、白介细胞素1β及白介细胞素6水平与腰椎和股骨骨密度均呈负相关(P<0.05)。结果说明,去卵巢大鼠骨组织中肿瘤坏死因子α、白介细胞素1β及白介细胞素6水平升高,破骨细胞占视野面积比例升高,增强破骨活动,骨小梁和成骨细胞占视野面积比例下降,加速骨吸收进程,导致形成骨质疏松。BACKGROUND： It has found that secretions of tumor necrosis factor α（TNF-α）, interleukin 1β（IL-1β） and interleukin 6（IL-6） are closely related to osteoporosis OBJECTIVE： To further investigate the levels of TNF-α, IL-1β and IL-6 of bone tissue in ovariectomized rats and their relationship with osteoporosis. METHODS： Sixty 3-month-old female Sprague-Dawley rats were randomly divided into control group and test group（n=30 per group）. In the test group osteoporosis model was induced by ovariectomy followed by intraperitoneal injection of aluminum nitride solution; while rats in the control group were given intraperitoneal injection of normal saline without ovariectomy. The experiment cycle was 12 weeks. One week after modeling, serum level of estradiol was tested, and the bone mineral density of rats measured with bone densitometry. In the meanwhile, the levels of TNF-α, IL-1β and IL-6 in bone tissue were detected to analyze the correlation between cytokines and bone mineral density. RESULTS AND CONCLUSION： The level of serum estradiol in the test group was significantly lower than that in the control group（P=0.007）; the bone mineral density of the lumbar spine and femur in the test group was significantly lower than that in the control group（P=0.006, 0.004）. Compared with the control group, the percentages of trabecular bone and osteoblast area within the visual field in the test group were significantly lower, while the osteoclast area within the visual field was significantly higher（P=0.037, 0.029, 0.044）. Compared with the control group, the levels of TNF-α, IL-1β and IL-6 in the test group significantly increased（P=0.032, 0.031, 0.025）. And the bone mineral density of the lumbar spine and femur was negatively correlated with the levels of TNF-α, IL-1β and IL-6 in the test group（P〈0.05）. In conclusion, the osteoclast activity is enhanced by elevating the levels of TNF-α, IL-1β and IL-6 of bone tissue of ovariectomized rats, and increasing the percentage

关 键 词：卵巢 细胞因子 骨质疏松 组织工程 组织构建 骨组织工程 骨组织 

分 类 号：R318[医药卫生—生物医学工程]