纳米羟基磷灰石/聚酰胺66盖髓对造牙本质细胞及微血管的影响  

Nano-hydroxyapatite/polyamide 66 as a direct pulp capping agent has effects on histological changes of odontoblasts and microvessels

作　　者：佟玮玮 王健平[1] 赵千宁 苏丹[1] 于彦衡 

机构地区：[1]佳木斯大学附属口腔医院,黑龙江省佳木斯市154002

出　　处：《中国组织工程研究》2016年第16期2418-2424,共7页Chinese Journal of Tissue Engineering Research

基　　金：2015年佳木斯大学研究生科技创出项目(LZZ2015-023)~~

摘　　要：背景:前期研究表明,纳米羟基磷灰石/聚酰胺66复合物具有用作根充材料的基本理化性能,对感染髓腔有抗菌性,对细胞无细胞毒性,但将其作为直接盖髓剂的研究还比较片面。目的:观察纳米羟基磷灰石/聚酰胺66复合物用作盖髓剂的组织学反应。方法:取Wistar大鼠12只,在上下颌第一、二臼齿做Ⅰ类洞至露髓,随机分3组,分别采用纳米羟基磷灰石基磷灰石、氢氧化钙盖髓,均以玻璃离子充填。充填后/聚酰胺66复合物、纳米羟7,30 d处死动物,采用血管墨汁灌注法观察牙本质细胞和牙髓微血管组织学变化。结果与结论:①充填后7 d:纳米羟基磷灰石/聚酰胺66复合物组牙髓纤维细胞增生,前期牙本质增厚,血管扩张明显;纳米羟基磷灰石组牙髓纤维细胞增生,前期牙本质无明显增厚,血管扩张;氢氧化钙组髓室大部牙髓组织坏死,下方牙髓纤维细胞增生明显,有一定炎症细胞浸润,血管坏死;②充填后30 d:纳米羟基磷灰石/聚酰胺66复合物组盖髓下方前期牙本质增厚明显,牙髓纤维细胞增生,成牙本质细胞增加,血管扩张;纳米羟基磷灰石组前期牙本质增厚,血管扩张明显;氢氧化钙组穿髓孔下牙髓表面坏死团块,牙髓交界处成牙本质细胞活跃牙本质壁上前期牙本质无明显增厚,坏死区下方血管密集;③结果表明:纳米羟基磷灰石/聚酰胺66复合物具有诱导牙髓细胞再生的作用。BACKGROUND： Previous studies have demonstrated that nano-hydroxyapatite/polyamide 66（n HA/PA66） composites as a root canal filling material possess the basic physical and chemical properties, have antibacterial properties against the infection of medullary cavity, and have no cytotoxicity. However, it is rarely reported as a direct pulp capping agent. OBJECTIVE： To observe the histological reaction of n HA/PA66 composites as the pulp capping agent. METHODS： The first and second molars from 12 Wistar rats were selected to prepare class I cavities by exposing the dental pulp. Then, these rats were randomized into three groups and subjected to dental pulp capping using nH A/PA66 composite, n HA and calcium hydroxide, respectively, followed by filling with glass ionomer cement. At 7 and 30 days after filling, histological changes of odontoblasts and microvessels in the dental pulp were observed using ink perfusion method. RESULTS AND CONCLUSION： At 7 days after filling, significant changes in dental pulp fibroblast proliferation, predentin thickening, dilation of blood vessels were observed in the n HA/PA66 group; only dental pulp fibroblast proliferation with no predentin thickening and dilation of blood vessels was found in the n HA group; in the calcium hydroxide group, the pulp tissues in the pulp chamber were mostly damaged, and obvious hyperplasia in dental pulp fibroblasts and certain inflammatory cell infiltration with vascular necrosis were observed. At 30 days after filling, predentin thickness was increased further, dental pulp fibroblasts proliferated, the number of odontoblasts increased, and dilation of blood vessels was observed in the n HA/PA66 group; predentin thickening and dilation of blood vessels were obviously visible in the n HA group; pulp necrosis was found on the pulp surface below the pitting pore, odontoblasts grew actively at the pulp junction, with no obvious thickening of the predentin, and there were dense vessels below the necrotic area. These findings indicate that the nH

关 键 词：牙髓复盖术 羟基磷灰石类 组织工程 生物材料 口腔生物材料 纳米羟基磷灰石/聚酰胺66复合物 纳米羟基磷灰石 直接盖髓 氢氧化钙 动物模型 牙本质细胞 微血管 墨汁灌注 

分 类 号：R318[医药卫生—生物医学工程]