VEGF抗体对高糖状态下人肾小球系膜细胞生长、增殖和迁徙的影响  

Influence of Anti- VEGF on Proliferation and Migration of High Glucose in Glomerular Mesangial Cells

作　　者：蒋晓立 陈学波[1] 吴广宇[1] 颜思诗[1] 顾琼英[1] 

机构地区：[1]浙江省舟山医院肾病科,舟山361032

出　　处：《中国中西医结合肾病杂志》2016年第3期215-217,I0003,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：舟山市科技局立项项目(No.2011c12043)

摘　　要：目的:明确VEGF抗体在人肾小球系膜细胞高糖状态模型生长、增殖和迁徙中的重要作用,为VEGF抗体应用于糖尿病肾病的临床治疗提供实验依据。方法:(1)建立人肾小球系膜细胞的高糖状态模型;(2)检测人肾小球系膜细胞高糖状态模型自分泌的VEGF;(3)观察VEGF抗体对人肾小球系膜细胞高糖状态模型生长、增殖和迁徙能力的影响。结果:(1)与对照组、低糖组比较,高糖组的肾小球系膜细胞分泌VEGF量最多,差异有统计学意义(P<0.05);(2)在人肾小球系膜细胞高糖状态模型的培养基中加入不同浓度的VEGF抗体,随VEGF抗体浓度升高,其细胞凋亡率、细胞增殖并非呈线性关系;(3)在人肾小球系膜细胞高糖状态模型中提取细胞,加入transwell上层小室,加入不同浓度的VEGF抗体,VEGF抗体浓度一定范围内增高会降低系膜细胞迁徙能力。结论:(1)高糖引起的肾小球系膜细胞VEGF表达上调,可能是糖尿病肾病的发病机制之一;(2)VEGF抗体促进肾小球系膜细胞的凋亡,降低细胞增殖能力与迁徙能力。Objective： We observed the expression of VEGF secretion,cell growth,proliferation and migration in human glomerular mesangial stimulated by high glucose; and the function of VEGF antibodies. Methods：（ 1） Establishment of human mesangial cells in high glucose condition model;（ 2） Detected in human mesangial cells high glucose condition model autocrine VEGF;（ 3） Observed effects of VEGF antibody human mesangial cell growth,proliferation and migration ability of models. Results：（ 1）VEGF respectively detected by ELISA obtained under high glucose stimulation,VEGF secretion highest,there was a significant statistical difference（ P〈0. 05） with the normal group;（ 2） With VEGF antibody concentration,at the same time stimulating their apoptosis rate is not a linear relationship,but the apoptosis rate increased initial concentration,but to a certain concentration（ 100 pg / ml）occurs when a plateau. Similarly,cell proliferation,apoptosis is also similar with the initial concentration with VEGF,decreased cell proliferation,but to a certain concentration（ 100 pg / ml）,cell proliferation is not the case there continues to decline;（ 3） The human mesangial cellshigh glucose conditionmodel added 8μm pore transwell upper chamber,while adding different concentrations of VEGF antibody,12 h after counting the number of cells into the lower chambers. The experiment used a three density levels found in the antibody concentration range increased VEGF reduces mesangial cell migration capacity. Conclusion：（ 1） VEGF expression in mesangial cells leads to upregulation of high- sugar,may be one of the pathogenesis of diabetic nephropathy;（ 2） VEGF antibodies induce apoptosis in glomerular mesangial cells,reducing cell proliferation and migration capabilities.

关 键 词：糖尿病肾病 肾小球系膜细胞 VEGF 高糖 

分 类 号：R587.2[医药卫生—内分泌]